Antibody:	Nell-1 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: enamel organ	Present		1A
TS21: tooth bud	Present		1A
TS21: tooth mesenchyme	Present	Regionally restricted	1A	Expression is in the mesenchyme underlying the enamel epithelium.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present		1B
TS22: dental papilla	Present		1B
TS22: dental sac	Present		1B
TS22: enamel knot	Present		1B
TS22: inner enamel epithelium	Present		1B
TS22: outer enamel epithelium	Present		1B
TS22: stellate reticulum	Present		1B

Structure	Level	Pattern	Image	Note
TS24: early bell stage tooth	Present		1D , 1C
TS24: inner enamel epithelium	Present		1D , 1C
TS24: outer enamel epithelium	Present		1D , 1C
TS24: secondary enamel knot	Present		1D , 1C
TS24: stratum intermedium	Present		1D , 1C
TS24: dental papilla	Present		1D , 1C	Expression is stronger at the dental cups.
TS24: stellate reticulum	Absent		1D , 1C

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Present	Regionally restricted	1F , 1E	Expression is in differentiating ameloblasts.
TS26: dental papilla	Present	Regionally restricted	1F , 1E	Expression is in differentiating odontoblasts and in the endothelial cells of blood vessels.
TS26: late bell stage tooth	Present		1E , 1F
TS26: outer enamel epithelium	Present		1E , 1F
TS26: stratum intermedium	Present		1E , 1F
TS26: blood vessel endothelium	Strong	Regionally restricted	1E , 1F	Expression is in the endothelial cells of blood vessels in dental papilla.

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Strong	Regionally restricted	2C , 2B , 2A	Expression is in secretory and mature ameloblasts and in immature enamel.
TS27: enamel	Strong	Regionally restricted	2A , 2B , 2C	Expression is in immature enamel.

Structure	Level	Pattern	Image	Note
TS28: enamel organ	Strong	Regionally restricted	2D , 2E , 2F	Expression is in secretory and mature ameloblasts and in immature enamel.
TS28: enamel	Strong	Regionally restricted	2F , 2E , 2D	Expression is in immature enamel.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Present		2I , 2G , 2H
TS28: enamel organ	Strong	Regionally restricted	2H , 2I , 2G	Expression is in secretory and mature ameloblasts and in immature enamel.
TS28: enamel	Strong	Regionally restricted	2I , 2H , 2G	Expression is in immature enamel.
TS28: dental papilla	Strong	Regionally restricted	2I , 2G , 2H	Expression is in odontoblasts including odontoblastic bodies and odontoblastic processes in dentinal tubules.

Structure	Level	Pattern	Image	Note
TS28: dental papilla	Present	Regionally restricted	3C , 3A , 3B	Expression is in odontoblasts and odontoblastic processes.
TS28: enamel organ	Present	Regionally restricted	3C , 3B , 3A	Expression is in the reduced dental epithelium.
TS28: enamel	Absent		3C , 3A , 3B

Structure	Level	Pattern	Image	Note
TS28: dental papilla	Present	Regionally restricted	3D , 3F , 3E	Expression is in odontoblastic bodies and odontoblastic processes and in endothelial cells of blood vessels.
TS28: blood vessel endothelium	Present		3E , 3F , 3D	Expression is in endothelial cells of blood vessels in the tooth.