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Gene Expression Data
Assay Details
Assay
Reference: J:301543 Li J, et al., Modeling by disruption and a selected-for partner for the nude locus. EMBO Rep. 2020 Dec 28;:e49804
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6693339
Gene symbol: Aff4
Gene name: AF4/FMR2 family, member 4
Antibody: anti-AFF4 (YK6)
Detection system: Secondary antibody coupled to DyLight 488
Assay notes: There were two anti-Aff4 antibodies in the paper. It was not specified which one was used.
Results
Specimen 3B/EV2A': postnatal day 9 (more )
Note: Double labeled: green - Aff4; red - Foxn1 (assay MGI:6693349).
Structure Level Pattern Image Note
TS28: hair shaft Present Regionally restricted EV2A' , 3B Expressed in the nuclei of epithelial cells in the differentiating cortex.

Specimen 3C/EV2B': embryonic day 16.5 (more )
Note: Double labeled: green - Aff4; red - Foxn1 (assay MGI:6693349).
Structure Level Pattern Image Note
TS24: epidermis Present Regionally restricted 3C , EV2B' Expressed in the differentiating layers. Virtually all Foxn1+ cells expressed Aff4, as there were Aff4+ only cells.

Specimen 3E/EV2D': postnatal day 0 (more )
Note: Double labeled: green - Aff4; red - Foxn1 (assay MGI:6693349).
Structure Level Pattern Image Note
TS27: thymus cortex epithelium Present EV2D' , 3E Virtually all Foxn1+ cells expressed Aff4, as there were Aff4+ only cells.
TS27: thymus medulla epithelium Present EV2D' , 3E Virtually all Foxn1+ cells expressed Aff4, as there were Aff4+ only cells.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory