Antibody:	Col27a1 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixative was 5% acetic acid, 95% ethanol.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong	Widespread	1A	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: foot cartilage condensation	Strong	Widespread	1A	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong	Widespread	1B	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: rib cartilage condensation	Strong	Widespread	1B	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: lung	Absent		1B

Structure	Level	Pattern	Image	Note
TS22: retina internal limiting lamina	Present		1C
TS22: cornea stroma	Weak		1C

Structure	Level	Pattern	Image	Note
TS26: stellate reticulum	Strong		1D

Structure	Level	Pattern	Image	Note
TS22: arterial blood vessel	Strong	Regionally restricted	2A	Expression is in the walls of the major arteries associated with the heart.

Structure	Level	Pattern	Image	Note
TS26: arterial blood vessel	Absent		2D

Structure	Level	Pattern	Image	Note
TS22: dermis	Strong		2F

Structure	Level	Pattern	Image	Note
TS26: dermis	Absent		2I

Structure	Level	Pattern	Image	Note
TS27: articular cartilage	Present	Regionally restricted	3A	Expression in the growth plate and articular cartilage is predominantly pericellular.
TS27: tibia	Present	Regionally restricted	3A	Expression in the growth plate and articular cartilage is predominantly pericellular.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	3G , 3D	Expression in the growth plate and articular cartilage is predominantly pericellular.
TS28: tibia	Present	Regionally restricted	3G , 3D	Expression in the growth plate and articular cartilage is predominantly pericellular.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Weak		S2B , S2A

Structure	Level	Pattern	Image	Note
TS20: cartilage	Weak		S2C , S2D

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong	Widespread	S3A	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: retina internal limiting lamina	Present		S3A
TS22: arterial blood vessel	Present	Regionally restricted	S3A	Expression is in the walls of the major arteries associated with the heart.
TS22: cornea stroma	Weak		S3A

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong	Widespread	S3B	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: retina internal limiting lamina	Present		S3B
TS22: arterial blood vessel	Present	Regionally restricted	S3B	Expression is in the walls of the major arteries associated with the heart.
TS22: cornea stroma	Weak		S3B

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong	Widespread	S3C	Expression is in the cartilaginous skeletal elements particularly the hypertrophic regions.
TS22: retina internal limiting lamina	Present		S3C
TS22: arterial blood vessel	Present	Regionally restricted	S3C	Expression is in the walls of the major arteries associated with the heart.
TS22: cornea stroma	Weak		S3C