Probe:

Gdf5 probe3

Structure	Level	Pattern	Image	Note
TS25: joint of auditory ossicle	Present	Regionally restricted	5F	Expression in annular ligament and incudostapedial joint.

Structure	Level	Pattern	Image	Note
TS25: incudomalleal joint	Present		5H	Expression in malleoincudal joint.

Structure	Level	Pattern	Image	Note
TS25: joint of auditory ossicle	Absent		5J	No evidence of joint-associated expression.

Structure	Level	Pattern	Image	Note
TS25: joint of auditory ossicle	Absent		5L	No evidence of joint-associated expression.

Structure	Level	Pattern	Image	Note
TS24: joint of auditory ossicle	Present	Regionally restricted	6B	Expression in annular ligament and stapedoincudal joint.
TS24: middle ear mesenchyme	Present	Regionally restricted	6B	Expression in some mesenchymal cells surrounding developing ossicles.

Structure	Level	Pattern	Image	Note
TS24: joint of auditory ossicle	Present		S2B	Expression similar to that in wild type.
TS24: middle ear mesenchyme	Present		S2B	Expression similar to that in wild type.

Structure	Level	Pattern	Image	Note
TS20: middle ear mesenchyme	Strong	Regionally restricted	7D	Highly expressed in area adjacent to styloid process. Expression in joint spaces among ossicles.