Antibody:	anti-Homer3 (NBP2-32607)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
Assay notes:	A tyramide amplification step was included to visualize proteins.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		S1F' , S1F	Proteins are enriched in neuronal processes.
TS28: hippocampus	Present		S1F , S1F'
TS28: brain blood vessel	Strong		S1F' , S1F	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present	Punctate	1C
TS20: jaw primordium mesenchyme	Present		1C	Expression is in the jaw mesenchyme, including the mesenchyme adjacent to the dental placode.
TS20: blood vessel endothelium	Strong		1C

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Present		1I	Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS21: molar mesenchyme	Present		1I

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present		1O
TS22: molar mesenchyme	Present		1O

Structure	Level	Pattern	Image	Note
TS27: molar dental papilla	Present	Regionally restricted	2F' , 2F	Expression is in odontoblasts.
TS27: molar enamel organ	Present	Regionally restricted	2F , 2F'	Expression is in differentiating ameloblasts.
TS27: blood vessel endothelium	Strong		2F , 2F'
TS27: alveolar process	Present	Regionally restricted	2F , 2F'	Expression is in osteoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3F	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: molar dental papilla	Present	Regionally restricted	3F	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Strong	Regionally restricted	3F	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3L	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: blood vessel endothelium	Strong	Regionally restricted	3L	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Present	Regionally restricted	3O	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Strong	Regionally restricted	3O	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: blood vessel endothelium	Strong	Regionally restricted	3R	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.
TS28: Hertwig's epithelial root sheath	Weak		3R

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4F
TS22: hippocampus	Present		4F
TS22: hypothalamus	Present		4F
TS22: neocortex	Present		4F
TS22: thalamus	Present		4F
TS22: trigeminal ganglion	Present		4F
TS22: forebrain ventricular layer	Present		4F	Expression is in the apical surface of the ventricular layer.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4I
TS22: hippocampus	Present		4I
TS22: neocortex	Present		4I
TS22: thalamus	Present		4I
TS22: trigeminal ganglion	Present		4I
TS22: forebrain ventricular layer	Present		4I''' , 4I'' , 4I , 4I'	Expression is in the apical surface of the ventricular layer.
TS22: choroid plexus	Present		4I' , 4I	Expression is in the apical surface.
TS22: hypothalamus	Present	Regionally restricted	4I'' , 4I	Expression is in the anterior hypothalamus concentrated in a few perinuclear puncta.
TS22: brain vascular element	Present	Regionally restricted	4I , 4I'''	Expression is in the endothelial lining of blood vessels in the brain, meninges, and choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: blood vessel endothelium	Strong		5F
TS22: olfactory nerve	Strong		5F
TS22: retina	Strong		5F
TS22: olfactory epithelium	Moderate		5F
TS22: trigeminal nerve	Moderate		5F
TS22: lens epithelium	Present		5F' , 5F
TS22: main olfactory bulb	Present		5F' , 5F
TS22: masseter	Present		5F' , 5F
TS22: skeletal muscle	Present		5F' , 5F
TS22: tongue muscle	Present		5F' , 5F
TS22: secondary palate	Present	Regionally restricted	5F , 5F'	Expression is in the medial epithelial seam.
TS22: palatal rugae	Present		5F , 5F'
TS22: submandibular gland primordium	Present	Regionally restricted	5F	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium epithelium	Present		5F	Expression is enriched in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	5I	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium	Present		5I	Expression is enriched in intracellular puncta.
TS22: blood vessel endothelium	Weak		5I

Structure	Level	Pattern	Image	Note
TS27: submandibular gland	Present	Regionally restricted	5L' , 5L	Expression is in the cytoplasm and cell membranes of acinar and duct cells.
TS27: blood vessel endothelium	Weak		5L , 5L'

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present		6C
TS27: olfactory epithelium	Present		6C	Expression is moderate at the apical surface.
TS27: blood vessel endothelium	Strong	Regionally restricted	6C	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.
TS27: lamina propria	Strong	Regionally restricted	6C	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.

Structure	Level	Pattern	Image	Note
TS27: nasal cavity respiratory epithelium	Present		6F	Cells of the respiratory epithelium had puncta enriched with proteins.
TS27: olfactory gland	Present	Regionally restricted	6F	Expression is in puncta in subsets of nasal gland acinar cells
TS27: blood vessel endothelium	Strong	Regionally restricted	6F	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.
TS27: lamina propria	Strong	Regionally restricted	6F	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	6L	Expression is in puncta at the apical surface of inner and outer hair cells and in perinuclear puncta in inner hair cells. Expression includes greater epithelial ridge cells, cells of Claudius and Deiter's cells.
TS27: stria vascularis	Present		6L
TS27: vestibular membrane	Present		6L

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present		6O
TS27: blood vessel endothelium	Strong		6O	Expression is in the vascular endothelium in the cochlea.

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Present	Regionally restricted	7F	Expression is in lingual epithelium, absent in taste buds.
TS22: tongue mesenchyme	Present	Regionally restricted	7F	Expression is in lingual mesenchyme.
TS22: blood vessel endothelium	Present		7F

Structure	Level	Pattern	Image	Note
TS27: taste bud	Weak		7L
TS27: tongue epithelium	Weak	Regionally restricted	7L	Expression is in lingual epithelium.
TS27: blood vessel endothelium	Present		7L

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	7O	Expression is in osteoblasts, osteoclasts, and osteocytes.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS20: jaw primordium mesenchyme	Moderate			There is immunostaining of the cytoplasm and plasma membranes.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS21: tooth mesenchyme	Weak			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: olfactory nerve	Strong
TS22: cranial nerve	Moderate
TS22: tooth epithelium	Strong			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS22: dental papilla	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS27: Hertwig's epithelial root sheath	Present
TS27: dental pulp	Present
TS27: inner enamel epithelium	Present
TS27: stratum intermedium	Present
TS27: dental papilla	Strong	Regionally restricted		Expression is in odontoblasts.
TS27: enamel organ	Moderate	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.

Structure	Level	Pattern	Image	Note
TS28: cranial nerve	Moderate
TS28: olfactory nerve	Moderate
TS28: Hertwig's epithelial root sheath	Present
TS28: dental pulp	Present
TS28: inner enamel epithelium	Present
TS28: stratum intermedium	Present
TS28: dental papilla	Strong	Regionally restricted		Expression is in odontoblasts.
TS28: enamel organ	Moderate	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.