Antibody:	anti-Homer2 (NBP1-85487)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
Assay notes:	A tyramide amplification step was included to visualize proteins.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Strong		S1E , S1E'	Proteins are enriched in neuronal processes.
TS28: hippocampus	Present		S1E' , S1E	Proteins are enriched in neuronal processes.
TS28: brain blood vessel	Moderate		S1E' , S1E	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present	Punctate	1B
TS20: jaw primordium mesenchyme	Present		1B	Expression is in the jaw mesenchyme, including the mesenchyme adjacent to the dental placode.
TS20: blood vessel endothelium	Moderate		1B

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Present		1H	Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS21: molar mesenchyme	Present		1H
TS21: blood vessel endothelium	Moderate		1H

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present		1N	Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS22: molar mesenchyme	Present		1N
TS22: blood vessel endothelium	Moderate		1N

Structure	Level	Pattern	Image	Note
TS27: molar dental papilla	Present	Regionally restricted	2E' , 2E	Expression is in odontoblasts.
TS27: molar enamel organ	Present	Regionally restricted	2E , 2E'	Expression is in differentiating ameloblasts.
TS27: stratum intermedium	Present		2E , 2E'
TS27: blood vessel endothelium	Moderate		2E , 2E'
TS27: alveolar process	Present	Regionally restricted	2E' , 2E	Expression is in osteoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3E	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: molar dental papilla	Present	Regionally restricted	3E	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Moderate	Regionally restricted	3E	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3K	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: blood vessel endothelium	Moderate	Regionally restricted	3K	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Present	Regionally restricted	3N	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Moderate	Regionally restricted	3N	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Strong		3Q
TS28: blood vessel endothelium	Moderate	Regionally restricted	3Q	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4E
TS22: hippocampus	Present		4E
TS22: hypothalamus	Present		4E
TS22: neocortex	Present		4E
TS22: thalamus	Present		4E
TS22: trigeminal ganglion	Present		4E
TS22: forebrain ventricular layer	Present		4E	Expression is in the apical surface of the ventricular layer.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4H
TS22: hippocampus	Present		4H
TS22: neocortex	Present		4H
TS22: thalamus	Present		4H
TS22: trigeminal ganglion	Present		4H
TS22: forebrain ventricular layer	Present		4H , 4H'' , 4H''' , 4H'	Expression is in the apical surface of the ventricular layer.
TS22: choroid plexus	Present		4H , 4H'	Expression is in the apical surface.
TS22: hypothalamus	Present	Regionally restricted	4H'' , 4H	Expression is in the anterior hypothalamus concentrated in perinuclear puncta.
TS22: brain vascular element	Moderate	Regionally restricted	4H''' , 4H	Expression is in the endothelial lining of blood vessels in the brain, meninges, and choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present		5E
TS22: main olfactory bulb	Present		5E
TS22: masseter	Present		5E
TS22: retina	Present		5E
TS22: skeletal muscle	Present		5E
TS22: secondary palate	Present	Regionally restricted	5E , 5E'	Expression is in the medial epithelial seam.
TS22: palatal rugae	Present		5E , 5E'
TS22: olfactory epithelium	Strong		5E' , 5E
TS22: olfactory nerve	Strong		5E' , 5E
TS22: trigeminal nerve	Strong		5E' , 5E
TS22: tongue muscle	Present		5E , 5E'	Expression is stronger in areas of differentiating muscle.
TS22: submandibular gland primordium	Present	Regionally restricted	5E	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium epithelium	Present		5E	Expression is enriched in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	5H	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium	Present		5H	Expression is enriched in intracellular puncta.
TS22: blood vessel endothelium	Weak		5H

Structure	Level	Pattern	Image	Note
TS27: submandibular gland	Present	Regionally restricted	5K' , 5K	Expression is in the cytoplasm and cell membranes of acinar and duct cells.
TS27: blood vessel endothelium	Weak		5K , 5K'

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present		6B
TS27: olfactory epithelium	Present		6B	Expression is strong at the apical surface.
TS27: blood vessel endothelium	Moderate	Regionally restricted	6B	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.
TS27: lamina propria	Moderate	Regionally restricted	6B	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.

Structure	Level	Pattern	Image	Note
TS27: nasal cavity respiratory epithelium	Present		6E	Cells of the respiratory epithelium had puncta enriched with proteins.
TS27: olfactory gland	Present	Regionally restricted	6E	Expression is in nasal gland acinar cells and were enriched in the apical membrane.
TS27: blood vessel endothelium	Moderate	Regionally restricted	6E	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.
TS27: lamina propria	Moderate	Regionally restricted	6E	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	6K	Expression is in stereocilia of inner and outer hair cells and are enriched in the apical surface and in perinuclear puncta and are also detectable in the cytoplasm. Expression includes greater epithelial ridge cells, cells of Claudius and Deiter's cells (weak).
TS27: stria vascularis	Present		6K
TS27: vestibular membrane	Present		6K

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present		6N
TS27: blood vessel endothelium	Moderate		6N	Expression is in the vascular endothelium in the cochlea.

Structure	Level	Pattern	Image	Note
TS22: tongue	Present	Regionally restricted	7E	Expression is in taste buds.
TS22: tongue epithelium	Present	Regionally restricted	7E	Expression is in lingual epithelium.
TS22: tongue muscle	Present		7E
TS22: fungiform papillae	Present	Regionally restricted	7E	Expression is in innervating nerves.

Structure	Level	Pattern	Image	Note
TS27: taste bud	Present		7K
TS27: tongue epithelium	Present	Regionally restricted	7K	Expression is in lingual epithelium.

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	7N	Expression is in osteoblasts, osteoclasts, and osteocytes.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS20: jaw primordium mesenchyme	Moderate			There is immunostaining of the cytoplasm and plasma membranes.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS21: tooth mesenchyme	Weak			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: cranial nerve	Strong
TS22: tooth epithelium	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS22: dental papilla	Weak			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS27: dental pulp	Weak
TS27: inner enamel epithelium	Weak
TS27: enamel organ	Strong	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.
TS27: stratum intermedium	Moderate
TS27: Hertwig's epithelial root sheath	Strong
TS27: dental papilla	Present	Regionally restricted		Expression is in odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: cranial nerve	Strong
TS28: dental pulp	Weak
TS28: inner enamel epithelium	Weak
TS28: enamel organ	Strong	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the pa
TS28: stratum intermedium	Moderate
TS28: Hertwig's epithelial root sheath	Strong
TS28: dental papilla	Strong	Regionally restricted		Expression is in odontoblasts.