About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:297761 Reibring CG, et al., Distinct and Overlapping Expression Patterns of the Homer Family of Scaffolding Proteins and Their Encoding Genes in Developing Murine Cephalic Tissues. Int J Mol Sci. 2020 Feb 13;21(4):1264
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6682020
Gene symbol: Homer2
Gene name: homer scaffolding protein 2
Antibody: anti-Homer2 (NBP1-85487)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
Assay notes: A tyramide amplification step was included to visualize proteins.
Results
Specimen S1E: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: hippocampus CA2 Strong S1E , S1E' Proteins are enriched in neuronal processes.
TS28: hippocampus Present S1E' , S1E Proteins are enriched in neuronal processes.
TS28: brain blood vessel Moderate S1E' , S1E Expression is in the endothelium.

Specimen 1B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: dental placode Present Punctate 1B
TS20: jaw primordium mesenchyme Present 1B Expression is in the jaw mesenchyme, including the mesenchyme adjacent to the dental placode.
TS20: blood vessel endothelium Moderate 1B

Specimen 1H: embryonic day 13.5 (more )
Note: Bud stage of tooth development.
Structure Level Pattern Image Note
TS21: molar epithelium Present 1H Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS21: molar mesenchyme Present 1H
TS21: blood vessel endothelium Moderate 1H

Specimen 1N: embryonic day 14.5 (more )
Note: Cap stage of tooth development.
Structure Level Pattern Image Note
TS22: molar epithelium Present 1N Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS22: molar mesenchyme Present 1N
TS22: blood vessel endothelium Moderate 1N

Specimen 2E: postnatal day 1 (more )
Note: Bell stage of tooth development.
Structure Level Pattern Image Note
TS27: molar dental papilla Present Regionally restricted 2E' , 2E Expression is in odontoblasts.
TS27: molar enamel organ Present Regionally restricted 2E , 2E' Expression is in differentiating ameloblasts.
TS27: stratum intermedium Present 2E , 2E'
TS27: blood vessel endothelium Moderate 2E , 2E'
TS27: alveolar process Present Regionally restricted 2E' , 2E Expression is in osteoblasts.

Specimen 3E: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 3E Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: molar dental papilla Present Regionally restricted 3E Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium Moderate Regionally restricted 3E Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Specimen 3K: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 3K Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: blood vessel endothelium Moderate Regionally restricted 3K Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Specimen 3N: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: molar dental papilla Present Regionally restricted 3N Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium Moderate Regionally restricted 3N Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Specimen 3Q: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: Hertwig's epithelial root sheath Strong 3Q
TS28: blood vessel endothelium Moderate Regionally restricted 3Q Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Specimen 4E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: corpus striatum Present 4E
TS22: hippocampus Present 4E
TS22: hypothalamus Present 4E
TS22: neocortex Present 4E
TS22: thalamus Present 4E
TS22: trigeminal ganglion Present 4E
TS22: forebrain ventricular layer Present 4E Expression is in the apical surface of the ventricular layer.

Specimen 4H: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: corpus striatum Present 4H
TS22: hippocampus Present 4H
TS22: neocortex Present 4H
TS22: thalamus Present 4H
TS22: trigeminal ganglion Present 4H
TS22: forebrain ventricular layer Present 4H , 4H'' , 4H''' , 4H' Expression is in the apical surface of the ventricular layer.
TS22: choroid plexus Present 4H , 4H' Expression is in the apical surface.
TS22: hypothalamus Present Regionally restricted 4H'' , 4H Expression is in the anterior hypothalamus concentrated in perinuclear puncta.
TS22: brain vascular element Moderate Regionally restricted 4H''' , 4H Expression is in the endothelial lining of blood vessels in the brain, meninges, and choroid plexus.

Specimen 5E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lens epithelium Present 5E
TS22: main olfactory bulb Present 5E
TS22: masseter Present 5E
TS22: retina Present 5E
TS22: skeletal muscle Present 5E
TS22: secondary palate Present Regionally restricted 5E , 5E' Expression is in the medial epithelial seam.
TS22: palatal rugae Present 5E , 5E'
TS22: olfactory epithelium Strong 5E' , 5E
TS22: olfactory nerve Strong 5E' , 5E
TS22: trigeminal nerve Strong 5E' , 5E
TS22: tongue muscle Present 5E , 5E' Expression is stronger in areas of differentiating muscle.
TS22: submandibular gland primordium Present Regionally restricted 5E Expression is in the nerves within the stroma.
TS22: submandibular gland primordium epithelium Present 5E Expression is enriched in intracellular puncta.

Specimen 5H: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: submandibular gland primordium epithelium Present Regionally restricted 5H Expression is in the nerves within the stroma.
TS22: submandibular gland primordium Present 5H Expression is enriched in intracellular puncta.
TS22: blood vessel endothelium Weak 5H

Specimen 5K: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: submandibular gland Present Regionally restricted 5K' , 5K Expression is in the cytoplasm and cell membranes of acinar and duct cells.
TS27: blood vessel endothelium Weak 5K , 5K'

Specimen 6B: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: olfactory nerve Present 6B
TS27: olfactory epithelium Present 6B Expression is strong at the apical surface.
TS27: blood vessel endothelium Moderate Regionally restricted 6B Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.
TS27: lamina propria Moderate Regionally restricted 6B Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.

Specimen 6E: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: nasal cavity respiratory epithelium Present 6E Cells of the respiratory epithelium had puncta enriched with proteins.
TS27: olfactory gland Present Regionally restricted 6E Expression is in nasal gland acinar cells and were enriched in the apical membrane.
TS27: blood vessel endothelium Moderate Regionally restricted 6E Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.
TS27: lamina propria Moderate Regionally restricted 6E Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.

Specimen 6K: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: organ of Corti Present Regionally restricted 6K Expression is in stereocilia of inner and outer hair cells and are enriched in the apical surface and in perinuclear puncta and are also detectable in the cytoplasm. Expression includes greater epithelial ridge cells, cells of Claudius and Deiter's cells (weak).
TS27: stria vascularis Present 6K
TS27: vestibular membrane Present 6K

Specimen 6N: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: cochlear ganglion Present 6N
TS27: blood vessel endothelium Moderate 6N Expression is in the vascular endothelium in the cochlea.

Specimen 7E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: tongue Present Regionally restricted 7E Expression is in taste buds.
TS22: tongue epithelium Present Regionally restricted 7E Expression is in lingual epithelium.
TS22: tongue muscle Present 7E
TS22: fungiform papillae Present Regionally restricted 7E Expression is in innervating nerves.

Specimen 7K: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: taste bud Present 7K
TS27: tongue epithelium Present Regionally restricted 7K Expression is in lingual epithelium.

Specimen 7N: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: alveolar process Present Regionally restricted 7N Expression is in osteoblasts, osteoclasts, and osteocytes.

Specimen Table S1 E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: dental placode Moderate There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS20: jaw primordium mesenchyme Moderate There is immunostaining of the cytoplasm and plasma membranes.

Specimen Table S1 Bud stage: embryonic day 13.5 (more )
Note: Bud stage of tooth development.
Structure Level Pattern Image Note
TS21: tooth epithelium Moderate There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS21: tooth mesenchyme Weak There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Specimen Table S1 Cap stage: embryonic day 14.5 (more )
Note: Cap stage of tooth development.
Structure Level Pattern Image Note
TS22: cranial nerve Strong
TS22: tooth epithelium Moderate There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS22: dental papilla Weak There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Specimen Table S1 Bell stage: postnatal day 1 (more )
Note: Bell stage of tooth development.
Structure Level Pattern Image Note
TS27: dental pulp Weak
TS27: inner enamel epithelium Weak
TS27: enamel organ Strong Regionally restricted Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.
TS27: stratum intermedium Moderate
TS27: Hertwig's epithelial root sheath Strong
TS27: dental papilla Present Regionally restricted Expression is in odontoblasts.

Specimen Table S1 P: postnatal (more )
Structure Level Pattern Image Note
TS28: cranial nerve Strong
TS28: dental pulp Weak
TS28: inner enamel epithelium Weak
TS28: enamel organ Strong Regionally restricted Expression is in differentiating, secretory, and maturation-stage ameloblasts and the pa
TS28: stratum intermedium Moderate
TS28: Hertwig's epithelial root sheath Strong
TS28: dental papilla Strong Regionally restricted Expression is in odontoblasts.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory