Antibody:	anti-Homer1 (12433-1-AP)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
Assay notes:	A tyramide amplification step was included to visualize proteins.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Strong		S1D' , S1D	Proteins are enriched in neuronal processes.
TS28: hippocampus	Present		S1D , S1D'	Proteins are enriched in neuronal processes.
TS28: brain blood vessel	Weak	Regionally restricted	S1D' , S1D	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Present	Punctate	1A
TS20: blood vessel endothelium	Weak		1A
TS20: jaw primordium mesenchyme	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: molar epithelium	Present		1G	Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS21: molar mesenchyme	Present		1G
TS21: blood vessel endothelium	Weak		1G

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present		1M	Subsets of cells of the dental epithelium have puncta enriched with proteins.
TS22: molar mesenchyme	Present		1M
TS22: blood vessel endothelium	Weak		1M

Structure	Level	Pattern	Image	Note
TS27: molar dental papilla	Present	Regionally restricted	2D' , 2D	Expression is in odontoblasts.
TS27: molar enamel organ	Present	Regionally restricted	2D , 2D'	Expression is in differentiating ameloblasts.
TS27: blood vessel endothelium	Weak		2D , 2D'
TS27: alveolar process	Present	Regionally restricted	2D' , 2D	Expression is in osteoblasts.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3D	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: molar dental papilla	Present	Regionally restricted	3D	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Weak	Regionally restricted	3D	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar enamel organ	Present	Regionally restricted	3J	Expression is in secretory ameloblasts, maturation-stage ameloblasts and cells of the papillary layer.
TS28: blood vessel endothelium	Weak	Regionally restricted	3J	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Present	Regionally restricted	3M	Expression is in odontoblasts, both young and old.
TS28: blood vessel endothelium	Weak	Regionally restricted	3M	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS28: Hertwig's epithelial root sheath	Strong		3P
TS28: blood vessel endothelium	Weak	Regionally restricted	3P	Expression is in the endothelium of blood vessels penetrating the papillary layer, the dental sac mesenchyme and the dental pulp.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4D
TS22: hippocampus	Present		4D
TS22: hypothalamus	Present		4D
TS22: neocortex	Present		4D
TS22: thalamus	Present		4D
TS22: trigeminal ganglion	Present		4D
TS22: forebrain ventricular layer	Present		4D	Expression is in the apical surface of the ventricular layer.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present		4G
TS22: hippocampus	Present		4G
TS22: neocortex	Present		4G
TS22: thalamus	Present		4G
TS22: trigeminal ganglion	Present		4G
TS22: forebrain ventricular layer	Present		4G , 4G' , 4G'' , 4G'''	Expression is in the apical surface of the ventricular layer.
TS22: choroid plexus	Present		4G , 4G'	Expression is in the apical surface.
TS22: hypothalamus	Present	Regionally restricted	4G , 4G''	Expression is in the anterior hypothalamus concentrated in perinuclear puncta.
TS22: brain vascular element	Weak	Regionally restricted	4G'' , 4G	Expression is in the endothelial lining of blood vessels in the brain, meninges, and choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present		5D
TS22: main olfactory bulb	Present		5D
TS22: retina	Present		5D
TS22: skeletal muscle	Present		5D
TS22: secondary palate	Present	Regionally restricted	5D , 5D'	Expression is in the medial epithelial seam.
TS22: masseter	Present		5D , 5D'
TS22: tongue muscle	Present		5D , 5D'
TS22: olfactory epithelium	Moderate		5D , 5D'
TS22: olfactory nerve	Moderate		5D , 5D'
TS22: trigeminal nerve	Moderate		5D , 5D'
TS22: palatal rugae	Strong		5D' , 5D
TS22: submandibular gland primordium	Present	Regionally restricted	5D	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium epithelium	Present		5D	Expression is enriched in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present	Regionally restricted	5G	Expression is in the nerves within the stroma.
TS22: submandibular gland primordium	Present		5G	Expression is enriched in intracellular puncta.
TS22: blood vessel endothelium	Weak		5G

Structure	Level	Pattern	Image	Note
TS27: submandibular gland	Present	Regionally restricted	5J' , 5J	Protein was concentrated in the apical surface as well as in puncta in acinar and duct cells.
TS27: blood vessel endothelium	Weak		5J' , 5J

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present		6A
TS27: olfactory epithelium	Present		6A	Expression is strong at the apical surface.
TS27: blood vessel endothelium	Weak	Regionally restricted	6A	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.
TS27: lamina propria	Weak	Regionally restricted	6A	Expression is in the endothelial lining of blood vessels within the lamina propria of the olfactory mucosa.

Structure	Level	Pattern	Image	Note
TS27: nasal cavity respiratory epithelium	Present		6D	Cells of the respiratory epithelium had puncta enriched with proteins.
TS27: olfactory gland	Present	Regionally restricted	6D	Expression is in nasal gland acinar cells and were enriched in the apical membrane.
TS27: blood vessel endothelium	Weak	Regionally restricted	6D	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.
TS27: lamina propria	Weak	Regionally restricted	6D	Expression is in the endothelial lining of blood vessels within the lamina propria of the respiratory mucosa.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	6J	Expression in inner and outer hair cells is enriched in the apical surface and in perinuclear puncta and are also detectable in the cytoplasm. Expression includes greater epithelial ridge cells, cells of Claudius and Deiter's cells.
TS27: stria vascularis	Present		6J
TS27: vestibular membrane	Present		6J
TS27: blood vessel endothelium	Weak		6J	Expression is in the vascular endothelium in the cochlea.
TS27: tectorial membrane	Absent		6J

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present		6M

Structure	Level	Pattern	Image	Note
TS22: tongue	Present	Regionally restricted	7D	Expression is in taste buds.
TS22: tongue epithelium	Present	Regionally restricted	7D	Expression is in lingual epithelium.
TS22: tongue mesenchyme	Present	Regionally restricted	7D	Expression is in lingual mesenchyme.
TS22: tongue muscle	Present		7D

Structure	Level	Pattern	Image	Note
TS27: fungiform papillae	Present	Regionally restricted	7J	Expression is in nerves innervating fungiform papillae.
TS27: taste bud	Present		7J

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present	Regionally restricted	7M	Expression is in osteoblasts and osteoclasts.

Structure	Level	Pattern	Image	Note
TS20: dental placode	Weak	Regionally restricted		Expression is strong in intracellular puncta.
TS20: jaw primordium mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS21: tooth mesenchyme	Weak			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS22: cranial nerve	Moderate
TS22: tooth epithelium	Moderate			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.
TS22: dental papilla	Weak			There is immunostaining of the cytoplasm and plasma membranes and strong immunostaining in intracellular puncta.

Structure	Level	Pattern	Image	Note
TS27: inner enamel epithelium	Weak
TS27: dental pulp	Weak
TS27: stratum intermedium	Weak
TS27: enamel organ	Strong	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.
TS27: dental papilla	Present	Regionally restricted		Expression is in odontoblasts.
TS27: Hertwig's epithelial root sheath	Strong

Structure	Level	Pattern	Image	Note
TS28: cranial nerve	Moderate
TS28: inner enamel epithelium	Weak
TS28: dental pulp	Weak
TS28: stratum intermedium	Weak
TS28: enamel organ	Strong	Regionally restricted		Expression is in differentiating, secretory, and maturation-stage ameloblasts and the papillary layer.
TS28: dental papilla	Present	Regionally restricted		Expression is in odontoblasts.
TS28: Hertwig's epithelial root sheath	Strong