Antibody:	CD2AP antibody (R1774)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	It was not specified which antibody was used, although it was mentioned that all three antisera gave similar staining patterns.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Weak	Regionally restricted	3A	Expressed in the cytoplasm of the primitive ectoderm cells surrounding the proamniotic cavity.

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		3A

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Weak		3B
TS10: embryo endoderm	Weak		3B
TS10: embryo mesoderm	Weak		3B

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		3B

Structure	Level	Pattern	Image	Note
TS18: trophoblast giant cell	Weak		3C
TS18: labyrinthine zone	Present	Regionally restricted	3C	Expressed in the endothelium.
TS18: spongiotrophoblast	Absent		3C

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		3D
TS28: endometrium epithelium	Present		3D
TS28: endometrium stroma	Absent		3D

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Diffuse	3E
TS28: ovary follicle cell	Present	Diffuse	3E
TS28: mesenchymal stroma of ovary	Absent		3E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatogonium		Moderate		3F
TS28: Leydig cell	Leydig cell	Moderate		3F
TS28: Sertoli cell	Sertoli cell	Moderate		3F
TS28: spermatid		Absent		3F
TS28: spermatocyte		Absent		3F

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4A
TS19: surface ectoderm	Present		4A
TS19: urogenital ridge	Present		4A
TS19: heart	Present		4A	somewhat weaker signal
TS19: liver	Present		4A	somewhat weaker signal
TS19: lung	Present		4A	somewhat weaker signal

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Strong		4B
TS19: nephric duct	Strong		4B
TS19: gonad	Present		4B

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Strong		4C

Structure	Level	Pattern	Image	Note
TS19: liver parenchyma	Moderate		4D

Structure	Level	Pattern	Image	Note
TS19: lobar bronchus epithelium	Strong		4E
TS19: pleura	Strong		4E
TS19: lung mesenchyme	Present		4E	a weaker signal

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Present		4F
TS19: pericardium	Present		4F
TS19: heart blood vessel	Present	Regionally restricted	4F	Expressed in the capillaries.
TS19: cardiac muscle tissue	Absent		4F

Structure	Level	Pattern	Image	Note
TS25: mesenchyme of metanephros	Present	Regionally restricted	5A	Expressed in the pretubular aggregating mesenchyme cells around the tips of the branching collecting duct. Expression in the undifferentiated metanephric mesenchyme cells was barely detectable.
TS25: pretubular aggregate	Present		5A
TS25: ureteric tip	Present		5A
TS25: collecting duct	Strong		5A

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Strong		5B
TS28: glomerular tuft	Strong		5B
TS28: renal distal tubule	Strong		5B
TS28: renal proximal tubule	Weak		5B	weak or negligible labelling

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present	Regionally restricted	5C	Expressed in the differentiating intestinal epithelium located in the crypts.
TS25: intestinal villus	Strong	Regionally restricted	5C	Expressed in the basal and middle regions of the villi. The signal in the tips of the villi was clearly weaker.
TS25: small intestine mucosa	Strong	Regionally restricted	5C	Expressed in the basal and middle regions of the villi. The signal in the tips of the villi was clearly weaker.

Structure	Level	Pattern	Image	Note
TS28: bile duct epithelium	Present		5E
TS28: hepatic vein	Present	Regionally restricted	5E	Expressed in the endothelial cells.
TS28: liver vasculature	Present	Regionally restricted	5E	Expressed in capillaries.
TS28: liver parenchyma	Absent		5E

Structure	Level	Pattern	Image	Note
TS25: stomach epithelium	Present	Uniform	5F	a rather uniform, diffuse signal

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present	Regionally restricted	5G	Expressed in the parietal cells as a weaker signal compared with that of chief cells and mucous neck cells.
TS28: stomach mucosa	Strong	Regionally restricted	5G	Expressed in the mucous neck cells and chief cells.

Structure	Level	Pattern	Image	Note
TS25: lobar bronchus epithelium	Strong		5H	distinct apical accumulation
TS25: alveolus epithelium	Absent		5H	Expression declined abruptly.
TS25: lung vascular element	Present		5H

Structure	Level	Pattern	Image	Note
TS18: inner ear	Strong	Regionally restricted	5I	Expression was prominent in the endolymphatic duct whereas the semicircular canals showed weak expression.

Structure	Level	Pattern	Image	Note
TS25: tooth epithelium	Strong	Regionally restricted	6A	Expressed in the epithelial ameloblasts.
TS25: stratum intermedium	Strong		6A
TS25: tooth mesenchyme	Strong	Regionally restricted	6A	Expressed strongly in the mesenchymal odontoblasts. The dental mesenchyme, giving rise to odontoblasts, showed a weaker labeling.
TS25: dental papilla	Absent		6A

Structure	Level	Pattern	Image	Note
TS25: 4th ventricle choroid plexus epithelium	Strong		6B
TS25: brain blood vessel	Present		6B
TS25: 4th ventricle ependyma	Absent		6B

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Present		6C	Expressed in the ganglion cells. Weaker or negligible labeling in the nerve fibers and satellite cells.

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present	Regionally restricted	6D	Expressed in the chondroblasts.

Structure	Level	Pattern	Image	Note
TS23: rib cartilage	Strong	Regionally restricted	6E	Expressed strongly in the rim of osteoblasts. Also expressed in the chondroblasts.

Structure	Level	Pattern	Image	Note
TS28: rib cartilage	Present	Regionally restricted	6F	Expressed in the osteocytes whereas the periosteal cells were negative.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Weak
TS12: embryo endoderm	Weak
TS12: embryo mesoderm	Weak
TS12: allantois	Present

Structure	Level	Pattern	Image	Note
TS28: decidua	Present

Structure	Level	Pattern	Image	Note
TS18: cartilage condensation	Present
TS18: dorsal root ganglion	Present
TS18: liver parenchyma	Present
TS18: mesonephric tubule	Present
TS18: nephric duct	Present
TS18: ureteric bud	Present
TS18: metanephric mesenchyme	Weak			Expressed in undifferentiated mesenchyme.
TS18: lung epithelium	Present	Regionally restricted		Expressed in bronchial epithelium.
TS18: lung	Weak	Regionally restricted		Expressed in parenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: mesenchyme of metanephros	Present	Regionally restricted		Expressed in aggregating mesenchyme.
TS23: bile duct epithelium	Present
TS23: choroid plexus	Present
TS23: collecting duct	Present
TS23: cortical early distal tubule	Present
TS23: dorsal root ganglion	Present
TS23: maturing glomerular tuft	Present
TS23: cortical early proximal tubule	Present	Regionally restricted		Expressed in proximal tubules in cortex, not in medulla.
TS23: metanephros epithelium	Present	Regionally restricted		Expressed in urothelium.
TS23: liver parenchyma	Weak
TS23: lung epithelium	Present	Regionally restricted		Expressed in the bronchial epithelium, not alveolar epithelium.
TS23: lung parenchyma	Present			Somewhat weaker expression in the parencymal cells.

Structure	Level	Pattern	Image	Note
TS25: bile duct epithelium	Present
TS25: choroid plexus	Present
TS25: conjunctival sac	Present
TS25: endolymphatic duct	Present
TS25: glomerular tuft	Present
TS25: lens epithelium	Present
TS25: pelvic urothelial lining	Present
TS25: renal distal tubule	Present
TS25: urothelium of ureter	Present
TS25: metanephros epithelium	Present	Regionally restricted		Expressed in urothelium.
TS25: liver parenchyma	Weak
TS25: bone	Present	Regionally restricted		Expressed in the osteoblasts and osteocytes.
TS25: rib cartilage	Present	Regionally restricted		Expressed in chondroblasts.
TS25: thymus	Present	Regionally restricted		Expressed in lymphocytes and epithelium.
TS25: brain blood vessel	Present	Regionally restricted		Expressed in endothelial cells.
TS25: brain ependyma	Absent
TS25: cornea	Absent
TS25: nerve	Absent
TS25: sclera	Absent

Structure	Level	Pattern	Image	Note
TS28: adrenal cortex	Present
TS28: blood vessel endothelium	Present
TS28: esophagus squamous epithelium	Present
TS28: hair follicle	Present
TS28: large intestine epithelium	Present
TS28: pancreatic duct	Present
TS28: pelvic urothelial lining	Present
TS28: urothelium of ureter	Present
TS28: adrenal medulla	Absent
TS28: cardiac muscle tissue	Absent
TS28: dermis	Absent
TS28: skeletal muscle	Absent
TS28: smooth muscle tissue	Absent
TS28: oral epithelium	Present			Expressed in squamous epithelium.
TS28: stomach mucosa	Present			Expressed in the chief cells.
TS28: small intestine epithelium	Present	Regionally restricted		Expressed in crypt epithelium, not in the tips of villi.
TS28: exocrine pancreas	Present	Regionally restricted		Expressed in most acinar cells.
TS28: salivary gland	Present	Regionally restricted		Expressed in mucous and serous acini.
TS28: thyroid gland follicle	Present	Regionally restricted		Expressed in the epithelium.
TS28: alveolus epithelium	Absent
TS28: bone	Present	Regionally restricted		Expressed in osteocytes.
TS28: cartilage	Present	Regionally restricted		Expressed in the chondrocytes.
TS28: lymph node	Present			Expressed in the lymphocytes.
TS28: spleen	Present			Expressed in the lymphocytes.
TS28: spleen vascular element	Present	Regionally restricted		Expressed in the endothelial cells.
TS28: small intestine mucosa	Strong	Regionally restricted		Expressed in the crypts and in the basal and middle region of the villi. The signal was less pronounced in the mature cells close to the tips of the villi.