Antibody:	anti-AMH/MIS (C-20; sc-6886)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present		1C

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present		1D

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		1E
TS22: rete testis		Absent		1E	The rete cords were Sox9-positive, Amh-negative.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		1F

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present		1G

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		1H

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present		2A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		2B
TS22: germ cell of testis		Absent		2B
TS22: rete testis		Absent		2B

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3B Laminin/AMH
TS21: rete testis	Absent		3B Laminin/AMH	Rete strands were immunonegative.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3C Laminin/AMH
TS21: rete testis	Absent		3C Laminin/AMH	Rete strands were immunonegative.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present		3E Laminin/AMH

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present	Regionally restricted	3F Laminin/AMH	In the middle of the testis, some connections between testis cords were fully or partially negative for Amh.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present		3G Laminin/AMH

Structure	Cell Type	Level	Pattern	Image	Note
TS22: primary sex cord		Present	Regionally restricted	5A upper , 5A lower	The connections between testis cords were fully immunonegative.
TS22: Sertoli cell	Sertoli cell	Present		5A upper , 5A lower

Structure	Cell Type	Level	Pattern	Image	Note
TS22: primary sex cord		Present	Regionally restricted	5B upper , 5B lower	The connections between testis cords were partially immunonegative.
TS22: Sertoli cell	Sertoli cell	Present		5B upper , 5B lower

Structure	Cell Type	Level	Pattern	Image	Note
TS22: primary sex cord		Present	Regionally restricted	5C lower , 5C upper	The connections between testis cords were immunopositive.
TS22: Sertoli cell	Sertoli cell	Present		5C upper , 5C lower

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		6A Amh , 6A Sox9/Amh
TS22: primary sex cord		Present	Regionally restricted	6A Amh , 6A Sox9/Amh	At the anterior pole, testis cord areas adjacent to the connection sites between testis cords lost Amh staining.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		6B Amh , 6B Sox9/Amh
TS22: primary sex cord		Present		6B Amh , 6B Sox9/Amh	In the middle of the testis, testis cords lost Amh staining in the connection sites.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		6C Sox9/Amh , 6C Amh
TS22: primary sex cord		Present	Regionally restricted	6C Sox9/Amh , 6C Amh	In the posterior pole of the testis, testis cords were Amh-positive in the connection sites.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		7B Laminin/AMH
TS24: seminiferous cord		Present		7B Laminin/AMH	Expression was detected in the testis cord. All testis cords became individual and were connected to rete cords by E16.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: primary sex cord		Present		8A left	Amh-positive cells were found co-expressing Pax8 at the mesonephric border of the testis at E14.5. These cells were also found to express Sox9.
TS22: Sertoli cell	Sertoli cell	Present		8A left

Structure	Cell Type	Level	Pattern	Image	Note
TS22: primary sex cord		Present	Regionally restricted	8A right	Amh-positive cells were found co-expressing Sox9 at the mesonephric border of the testis at E14.5. These cells were also found to express Pax8.
TS22: Sertoli cell	Sertoli cell	Present		8A right

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		8B left
TS22: rete testis		Absent		8B left	Amh-negative cells that were Dmrt1-positive were located in rete cords. These cells were also Sox9-positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		8B right
TS22: rete testis		Absent		8B right	Amh-negative cells that were Sox9-positive were located in rete cords. These cells were also Dmrt1-positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: seminiferous cord		Present		8C left	Amh-positive cells were found co-expressing Pax8 at the border between testis cords and the rete testis at E16.5. These cells were also found to express Sox9.
TS24: Sertoli cell	Sertoli cell	Present		8C left

Structure	Cell Type	Level	Pattern	Image	Note
TS24: seminiferous cord		Present		8C right	Amh-positive cells were found co-expressing Sox9 at the border between testis cords and the rete testis at E16.5. These cells were also found to express Pax8.
TS24: Sertoli cell	Sertoli cell	Present		8C right

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		8D left
TS24: rete testis		Absent		8D left	Amh-negative cells that were Dmrt1-positive were located in rete cords. These cells were also Sox9-positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Sertoli cell	Sertoli cell	Present		8D right
TS24: rete testis		Absent		8D right	Amh-negative cells that were Sox9-positive were located in rete cords. These cells were also Dmrt1-positive.