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Gene Expression Data
Assay Details
Assay
Reference: J:297658 Kulibin AY, et al., Formation of the rete testis during mouse embryonic development. Dev Dyn. 2020 Aug 27;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6514865
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: Anti-Sox9 (AB5535)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present 1A
TS20: mesonephros mesenchyme Present Single cells 1A
TS20: mesonephric tubule Absent 1A
Specimen 1B: embryonic day 11.75 (more )
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 1B Expression was detected in a cluster near the anterior pole of the testis.
Specimen 1C: embryonic day 12.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS21: rete testis Present 1C
TS21: Sertoli cell Sertoli cell Present 1C
Specimen 1D: embryonic day 13.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS21: rete testis Present 1D Intensity of staining became higher at E13.5.
TS21: Sertoli cell Sertoli cell Present 1D
Specimen 1E: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS22: rete testis Present Regionally restricted 1E Expression was detected in the rete cords, which were Sox9-postive, Amh-negative.
TS22: Sertoli cell Sertoli cell Present 1E
Specimen 1F: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Level Pattern Image Note
TS24: rete testis Present 1F Expression was detected in the rete cords.
Specimen 1G: embryonic day 12.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); green - Pax8 (assay MGI:6514887).
Structure Cell Type Level Pattern Image Note
TS21: rete testis Present 1G
TS21: Sertoli cell Sertoli cell Present 1G
Specimen 2A: embryonic day 13.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); green - Cdh1 (assay MGI:6515565).
Structure Level Pattern Image Note
TS21: rete testis Present 2A
Specimen 2B: embryonic day 14.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); green - Cdh1 (assay MGI:6515565).
Structure Level Pattern Image Note
TS22: rete testis Present 2B
TS22: germ cell of testis Absent 2B
Specimen 6A: embryonic day 14.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); yellow - basement membrane (labeled by anti-mouse secondary antibody). Anterior pole of the gonad.
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 6A Sox9/Amh , 6A Sox9
TS22: rete testis Present Regionally restricted 6A Sox9 , 6A Sox9/Amh Expression was detected in rete cords at the anterior pole.
Specimen 6B: embryonic day 14.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); yellow - basement membrane (labeled by anti-mouse secondary antibody). Middle of the gonad.
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 6B Sox9 , 6B Sox9/Amh
Specimen 6C: embryonic day 14.5 (more )
Note: Triple labeled: red - Sox9; white - Amh (assay MGI:6514880); yellow - basement membrane (labeled by anti-mouse secondary antibody). Posterior pole of the gonad.
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 6C Sox9/Amh , 6C Sox9
Specimen 8A right: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS22: rete testis Present 8A right
TS22: Sertoli cell Sertoli cell Present 8A right
TS22: primary sex cord Present Regionally restricted 8A right Sox9-positive cells were found co-expressing Amh at the mesonephric border of the testis at E14.5.
Specimen 8B right: embryonic day 14.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 8B right
TS22: rete testis Present 8B right The rete cords were Sox9-positive, Amh-negative.
Specimen 8C right: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS24: rete testis Present 8C right
TS24: Sertoli cell Sertoli cell Present 8C right
TS24: seminiferous cord Present Regionally restricted 8C right Sox9-positive cells were found co-expressing Amh at the border between testis cords and the rete testis at E16.5.
Specimen 8D right: embryonic day 16.5 (more )
Note: Double labeled: red - Sox9; white - Amh (assay MGI:6514880).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 8D right
TS24: rete testis Present 8D right The rete cords were Sox9-positive, Amh-negative.
Specimen 9A: embryonic day 12.5 (more )
Note: Double labeled: red - Sox9; white - Foxl2 (assay MGI:6515597).
Structure Level Pattern Image Note
TS20: rete ovarii of mesonephros Present 9A
Specimen 9B: embryonic day 13.5 (more )
Note: Double labeled: red - Sox9; white - Foxl2 (assay MGI:6515597).
Structure Level Pattern Image Note
TS21: rete ovarii of mesonephros Present 9B
Specimen 9C lower: embryonic day 13.5 (more )
Note: Double labeled: red - Sox9; white - Foxl2 (assay MGI:6515597).
Structure Level Pattern Image Note
TS21: rete ovarii of mesonephros Present 9C1 middle , 9C lower Some Sox9-positive cells close to the gonad tissue also expressed Foxl2. These cells also expressed Pax8.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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