Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96395
Lee S, et al., Essential role for the Prader-Willi syndrome protein necdin in axonal outgrowth. Hum Mol Genet. 2005 Mar 1;14(5):627-37
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6514724
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-Th
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
8B:
embryonic day 15.5
(more  )
Specimen
8B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Present |
Regionally restricted |
8B
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Expression was detected in the nigrostriatal pathway.
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Specimen
8B':
embryonic day 15.5; Ndntm2Stw/Ndn +
(more )
Note: Specimen carried a paternally inherited necdin deficiency and was functionally necdin null.
Specimen
8B':
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Ndntm2Stw/Ndn+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen carried a paternally inherited necdin deficiency and was functionally necdin null.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Present |
Regionally restricted |
8B'
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A severe disruption of the immunopositive projections in the nigrostriatal pathway and caudoputamen was noted.
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Specimen
8C:
embryonic day 15.5
(more )
Note: Section was ~600 um rostral to that in 8B.
Specimen
8C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section was ~600 um rostral to that in 8B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Present |
Regionally restricted |
8C
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Expression was detected in the nigrostriatal pathway.
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Specimen
8C':
embryonic day 15.5; Ndntm2Stw/Ndn +
(more )
Note: Specimen carried a paternally inherited necdin deficiency and was functionally necdin null. Section was ~600 um rostral to that in 8B'.
Specimen
8C':
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Ndntm2Stw/Ndn+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Specimen carried a paternally inherited necdin deficiency and was functionally necdin null. Section was ~600 um rostral to that in 8B'.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Trace |
Regionally restricted |
8C'
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Few immunopositive axons were noted in the necdin null more rostral section.
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Specimen
8D:
embryonic day 15.5
(more )
Note: Section was ~600 um caudal to that in 8B.
Specimen
8D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section was ~600 um caudal to that in 8B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Present |
Regionally restricted |
8D
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Expression was detected in the nigrostriatal pathway.
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| TS23: hypothalamus |
Present |
Regionally restricted |
8D
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Expression was detected in the dorsal medial hypothalamus.
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Specimen
8D':
embryonic day 15.5; Ndntm2Stw/Ndn +
(more )
Note: Specimen carried a paternally inherited necdin deficiency and was functionally necdin null. Section was ~600 um caudal to that in 8B'.
Specimen
8D':
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Ndntm2Stw/Ndn+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Specimen carried a paternally inherited necdin deficiency and was functionally necdin null. Section was ~600 um caudal to that in 8B'.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: caudate-putamen |
Present |
Regionally restricted |
8D'
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The more caudal necdin null section had fairly normal staining of the nigrostriatal pathway.
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| TS23: hypothalamus |
Present |
Regionally restricted |
8D'
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Fairly normal immunostaining was noted in the dorsal medial hypothalamus.
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