Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Sncgem1(flpo)Tasic
Name:	synuclein, gamma; endonuclease-mediated mutation 1, Bosiljka Tasic
MGI ID:	MGI:6514365
Synonyms:	Sncgem1(flpo)Hze
Gene:	Sncg  Location: Chr14:34092231-34096626 bp, - strand  Genetic Position: Chr14, 20.81 cM, cytoband B-C
Alliance:	Sncgem1(flpo)Tasic page

Germline Transmission:	Earliest citation of germline transmission: J:313881
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Sncgem1(flpo)Tasic expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Sncg (MGI:1298397)   Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence, a FlpO recombinase gene, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site following the STOP codon. The guide vector contained the Sncg guide RNA sequences designed for the endogenous stop codon and also a DNA sequence encoding a human codon-optimized Streptococcus pyogenes CRISPR associated protein 9 (SpCas9). Of note, the guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome (J:313881)

Activity:	Tissue activity of this recombinase allele
Driver:	Sncg (mouse) Summary of all recombinase alleles driven by Sncg. Alliance mouse Sncg gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sncg Mutation:	14 strains or lines available

Original:	J:313881 Lee BR, et al., Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization. Elife. 2021 Aug 13;10:e65482
All:	1 reference(s)