Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Ctxn3em1(flpo)Tasic
Name:	cortexin 3; endonuclease-mediated mutation 1, Bosiljka Tasic
MGI ID:	MGI:6514362
Synonyms:	Ctxn3em1(flpo)Hze
Gene:	Ctxn3  Location: Chr18:57601588-57611205 bp, + strand  Genetic Position: Chr18, 32.15 cM
Alliance:	Ctxn3em1(flpo)Tasic page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Ctxn3em1(flpo)Tasic expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Ctxn3 (MGI:3642816)   Mutation details: CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence, a FlpO recombinase gene, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site immediately following the STOP codon. The guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome. (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Ctxn3 (mouse) Summary of all recombinase alleles driven by Ctxn3. Alliance mouse Ctxn3 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ctxn3 Mutation:	6 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	2 reference(s)