Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Tardbpem2.1(TARDBP)H
Name:	TAR DNA binding protein; endonuclease-mediated mutation 2.1, Harwell
MGI ID:	MGI:6513996
Synonyms:	TARDBP (A02) EM Flp excised
Gene:	Tardbp  Location: Chr4:148696839-148711476 bp, - strand  Genetic Position: Chr4, 78.77 cM
Alliance:	Tardbpem2.1(TARDBP)H page

Germline Transmission:	Earliest citation of germline transmission: J:342505
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout) Mutations:    Insertion, Intragenic deletion   Tardbpem2.1(TARDBP)H expresses 1 gene Knock-in expresses: Organism Expressed Gene Homolog in Mouse Note human TARDBP (23435) Tardbp  (MGI:2387629)   Mutation details: A BAC targeting construct harbouring the mouse Tardpb locus (BAC B6Ng01-103M13) was engineered to replace the gene from the ATG start site to the TAG stop codon with the orthologous human TARDBP genomic sequence derived from a BAC with the human TARDPB locus (BAC RP11-829B14), and an FRT-flanked PGK/gb2/Neo cassette was inserted into intron 3 of the human gene. Flp-mediated recombination removed the selection cassette. (J:342505)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tardbp Mutation:	60 strains or lines available

Original:	J:342505 Devoy A, et al., Generation and analysis of innovative genomically humanized knockin SOD1, TARDBP (TDP-43), and FUS mouse models. iScience. 2021 Dec 17;24(12):103463
All:	2 reference(s)