Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:190636
Wiese CB, et al., A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract. Front Neurosci. 2012;6:130
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6513210
|
Gene symbol:
|
Ndrg4
|
Gene name:
|
N-myc downstream regulated gene 4
|
Probe:
|
MTF#968
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
6 - Ndrg4:
embryonic day 15.5
(more )
Specimen
6 - Ndrg4:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pelvic ganglion |
Strong |
Regionally restricted |
6_Ndrg4
|
Very highly localized expression is observed in two oval domains internal to the pelvic ganglia; these are either parallel to the axis of the bladder neck (horizontal) or vertically aligned to the urethral epithelium (vertical).
|
Specimen
8D- Ndrg4:
embryonic day 15.5
(more )
Specimen
8D- Ndrg4:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pelvic ganglion |
Strong |
Regionally restricted |
8D Ndrg4
|
Expression is seen in proximity to the nephric duct and ureter insertions sites where the bladder neck joins the urethra.
|
Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: nervous system |
Strong |
|
|
Expression is observed in aspects of the developing nervous system other than the pelvic ganglia.
|
Specimen
Table 1:
embryonic day 15.5
(more )
Specimen
Table 1:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pelvic ganglion |
Present |
|
|
Both sexes were examined; no reproducible differences were detected between them.
|
|