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Gene Expression Data
Assay Details
Assay
Reference: J:80044 Sohn SJ, et al., ERK5 MAPK regulates embryonic angiogenesis and acts as a hypoxia-sensitive repressor of vascular endothelial growth factor expression. J Biol Chem. 2002 Nov 8;277(45):43344-51
Assay type: RT-PCR
MGI Accession ID: MGI:6513156
Gene symbol: Angpt2
Gene name: angiopoietin 2
Probe: Ang2 1697F; Ang2 1747R
Probe preparation: labelled with SYBR green
Assay notes: Semi-quantitative RT-PCR. Standard curve was generated for the primer set with cDNA templates from ERK5+/- or ERK5-/- controls. The relative abundance of transcripts in unknown samples was determined by regression analyses based on these standard curves and was normalized against gamma-actin. Values were plotted as -fold over control Erk5+/- values.
Results
Image: 6B Ang2 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
ERK5-/- #1 E9.5 TS15: embryo Present 1 µg; total RNA involves: C57BL/6 Mapk7tm1Wnt/Mapk7tm1WntNot Specified
ERK5-/- #2 E9.5 TS15: embryo Present 1 µg; total RNA involves: C57BL/6 Mapk7tm1Wnt/Mapk7tm1WntNot Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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