Map2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:296029 Zhou Z, et al., CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity. Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22193-22203
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6512958
Gene symbol:	Map2
Gene name:	microtubule-associated protein 2

Antibody:	anti-MAP2 (2a+2b; clone AP-20)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. MAP2 antibody (H-300, SC-20172) MGI:6512918 may have been used in this assay.

Results

1D Map2 CAMSAP1 ©

Specimen 1D Map2 (left): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP1	Expression in dendrites.

1D Map2 CAMSAP2 ©

Specimen 1D Map2 (middle): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap2 (assay MGI:6512956); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP2	Expression in dendrites.

1D Map2 CAMSAP3 ©

Specimen 1D Map2 (right): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap3 (assay MGI:6512957); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP3	Expression in dendrites.

3C (+/+) merge ©

Specimen 3C (+/+): embryonic day 18.5 (more

)
Note: Triple labeled: green - Map2; red - BrdU; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		3C (+/+) merge

3C (-/-) Merge ©

Specimen 3C (-/-): embryonic day 18.5; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)
Note: Triple labeled: green - Map2; red - BrdU; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		3C (-/-) Merge

S1F (merge) ©

Specimen S1F: embryonic day 15.5 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		S1F (merge)

S1G (merge) ©

Specimen S1G: embryonic day 17.5 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		S1G (merge)

S1H (merge) ©

Specimen S1H: postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		S1H (merge)

S4E (+/+) (merge) ©

S4E (+/+) Map2 ©

Specimen S4E (+/+): embryonic day 18.0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		S4E (+/+) Map2 , S4E (+/+) (merge)

Specimen S4E (-/-): embryonic day 18.0; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		S4E (-/-) (merge) , S4E (-/-) Map2

Specimen S7A (+/+): postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		S7A (+/+) Map2 , S7A (+/+) Merged

Specimen S7A (-/-): postnatal day 0; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	S7A (-/-) Merged , S7A (-/-) Map2	Expression in cortical lamina fluctuated less than in (+/+) mice

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23