Gene Expression Data
Assay Details
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Assay
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Reference:
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J:276901
Taroc EZM, et al., GnRH-1 Neural Migration From the Nose to the Brain Is Independent From Slit2, Robo3 and NELL2 Signaling. Front Cell Neurosci. 2019;13:70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6512001
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Results
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Specimen
2A:
embryonic day 14.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
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2A
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| TS22: forebrain |
Present |
Regionally restricted |
2A
,
2A1
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Migrating immunopositive neurons made a sharp turn towards the basal forebrain.
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Specimen
2B:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Nell2tm1.2Matl/Nell2tm1.2Matl
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
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2B
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There was a small increase in the number of immunoreactive cells in the nasal area of Nell2-/- mutants.
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| TS22: forebrain |
Present |
Regionally restricted |
2B1
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2B
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No significant differences in the number of cells in the brain in the mutant compared to wild type. Migrating immunopositive neurons made a sharp turn towards the basal forebrain in mutant, as in wild type.
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Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
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3A
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| TS22: forebrain |
Present |
Regionally restricted |
3A1
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3A
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Migrating immunopositive neurons made a sharp turn towards the basal forebrain.
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Specimen
3B:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Robo3tm1Matl/Robo3tm1Matl
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
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3B
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There was no difference in total immunopositive cell number in the nasal area of the mutant.
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| TS22: forebrain |
Present |
Regionally restricted |
3B1
,
3B
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Migrating immunopositive neurons made a sharp turn towards the basal forebrain.
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Specimen
4A:
embryonic day 15.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basal forebrain |
Present |
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4A
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| TS23: forebrain |
Present |
Regionally restricted |
4A
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Immunopositive cells along the migratory track at the forebrain junction entry point, through the basal forebrain, and towards the preoptic area.
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Specimen
4B:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Nell2tm1.2Matl/Nell2tm1.2Matl
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basal forebrain |
Present |
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4B
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| TS23: forebrain |
Present |
Regionally restricted |
4B
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Immunopositive cells along the migratory track at the forebrain junction entry point, through the basal forebrain, and towards the preoptic area. There were no significant differences compared with controls.
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Specimen
4C:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Nell2tm1.2Matl/Nell2tm1.2Matl
Robo3tm1Matl/Robo3+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basal forebrain |
Present |
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4C
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| TS23: forebrain |
Present |
Regionally restricted |
4C
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Immunopositive cells along the migratory track at the forebrain junction entry point, through the basal forebrain, and towards the preoptic area. There were no significant differences compared with controls.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Mutant Allele(s):
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Slit2tm1Matl/Slit2tm1Matl
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forebrain |
Present |
Regionally restricted |
4D
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Immunopositive cells along the migratory track migrated more dorsally compared with wild type.
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Specimen
5C:
embryonic day 17.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: forebrain |
Present |
Regionally restricted |
5C
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Immunopositive cells along the migratory track.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Mutant Allele(s):
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Slit2tm1Matl/Slit2tm1Matl
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: forebrain |
Present |
Regionally restricted |
5D
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Immunopositive neurons migrating dorsally towards the cortical region in the Slit2-/- mutant.
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Specimen
5H:
embryonic day 17.5
(more )
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Mutant Allele(s):
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Slit2tm1Matl/Slit2tm1Matl
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: medial preoptic area |
Present |
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5I
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Immunoreactivity in the medial preoptic area in Slit2-/- mutant was comparable to wild type.
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Analysis Tools