Antibody:	anti-Collagen IV (ab6586)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present	Regionally restricted	S7page1-A Ctrl 7.5 , S7page1_Aa , S7page1_Ab	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS11: amnion mesoderm	Present	Regionally restricted	S7page1-A Ctrl 7.5 , S7page1_Aa , S7page1_Ab	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS11: extracellular matrix	Present	Regionally restricted	S7page1-A Ctrl 7.5 , S7page1_Aa , S7page1_Ab	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present	Regionally restricted	S7page1_Ab' , S7page1_A KO , S7page1_Aa'	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS11: amnion mesoderm	Present	Regionally restricted	S7page1_Ab' , S7page1_A KO , S7page1_Aa'	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS11: extracellular matrix	Present	Regionally restricted	S7page1_Ab' , S7page1_A KO , S7page1_Aa'	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present	Regionally restricted	S7page1_A Ctrl bottom , S7page1_A Ctrl bottom magnified	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS11: amnion mesoderm	Present	Regionally restricted	S7page1_A Ctrl bottom , S7page1_A Ctrl bottom magnified	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS11: extracellular matrix	Present	Regionally restricted	S7page1_A Ctrl bottom , S7page1_A Ctrl bottom magnified	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.

Structure	Level	Pattern	Image	Note
TS11: amnion ectoderm	Present	Regionally restricted	S7page1_A KO magnified , S7page1_A KO bottom	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS11: amnion mesoderm	Present	Regionally restricted	S7page1_A KO magnified , S7page1_A KO bottom	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS11: extracellular matrix	Present	Regionally restricted	S7page1_A KO magnified , S7page1_A KO bottom	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.

Structure	Level	Pattern	Image	Note
TS13: amnion ectoderm	Present	Regionally restricted	S7page1_B Ctrl top , S7page1_B Ctrl bottom	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS13: amnion mesenchyme	Present	Regionally restricted	S7page1_B Ctrl top , S7page1_B Ctrl bottom	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.
TS13: extracellular matrix	Present	Regionally restricted	S7page1_B Ctrl top , S7page1_B Ctrl bottom	Expression is uniform in the extracellular matrix between amniotic ectoderm and amniotic mesoderm cells.

Structure	Level	Pattern	Image	Note
TS13: amnion ectoderm	Present	Regionally restricted	S7page1_B KO bottom , S7page1_B KO top	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS13: amnion mesenchyme	Present	Regionally restricted	S7page1_B KO bottom , S7page1_B KO top	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.
TS13: extracellular matrix	Present	Regionally restricted	S7page1_B KO bottom , S7page1_B KO top	In this mutant expression is similar to control in regions of smooth amnion and is absent between the ectodermal cells in the regions with thickened amniotic ectoderm.