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Gene Expression Data
Assay Details
Assay
Reference: J:263987 Dobreva MP, et al., Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling. Development. 2018 Jul 2;145(13):dev157222
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6510822
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 antibody (ab19857)
Detection system: Secondary antibody
Results
Specimen 6B Ctrl left: embryonic day 7.5 (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B Ctrl left
Specimen 6B Ctrl middle: embryonic day 7.5 (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B Ctrl middle
Specimen 6B Ctrl right: embryonic day 7.5 (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B Ctrl right
Specimen 6B KO-SetA left: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B KO-SetA left About 80% of the mutants have expression similar to control embryos (shown).
Specimen 6B KO-SetA middle: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B KO-SetA middle About 80% of the mutants have expression similar to control embryos (shown).
Specimen 6B KO-SetA right: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present 6B KO-SetA right About 80% of the mutants have expression similar to control embryos (shown).
Specimen 6B KO-SetB left: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present Regionally restricted 6B KO-SetB left bottom , 6B KO-SetB left About 20% of the mutants have a negative area of expression in a portion of ectoderm, continuous with the amniotic ectoderm (shown).
Specimen 6B KO-SetB middle: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present Regionally restricted 6B KO-SetB middle , 6B KO-SetB middle bottom About 20% of the mutants have a negative area of expression in a portion of ectoderm, continuous with the amniotic ectoderm (shown).
Specimen 6B KO-SetB right: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: green - Pou5f1; red - Elf5 (assay MGI:6510818).
Structure Level Pattern Image Note
TS11: amnion ectoderm Present Regionally restricted 6B KO-SetB right bottom , 6B KO-SetB right About 20% of the mutants have a negative area of expression in a portion of ectoderm, continuous with the amniotic ectoderm (shown).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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