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Gene Expression Data
Assay Details
Assay
Reference: J:263987 Dobreva MP, et al., Amniotic ectoderm expansion in mouse occurs via distinct modes and requires SMAD5-mediated signalling. Development. 2018 Jul 2;145(13):dev157222
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6510818
Gene symbol: Elf5
Gene name: E74-like factor 5
Antibody: anti-Elf5 (sc-9645)
Detection system: Secondary antibody
Results
Specimen 6B Ctrl left: embryonic day 7.5 (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B Ctrl left
TS11: extraembryonic ectoderm Present 6B Ctrl left
TS11: amnion ectoderm Absent 6B Ctrl left
Specimen 6B Ctrl middle: embryonic day 7.5 (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B Ctrl middle
TS11: extraembryonic ectoderm Present 6B Ctrl middle
TS11: amnion ectoderm Absent 6B Ctrl middle
Specimen 6B Ctrl right: embryonic day 7.5 (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B Ctrl right
TS11: extraembryonic ectoderm Present 6B Ctrl right
TS11: amnion ectoderm Absent 6B Ctrl right
Specimen 6B KO-SetA left: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetA left 80% of Smad5 knockout embryos have expression similar to wild-type.
Specimen 6B KO-SetA middle: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetA middle 80% of Smad5 knockout embryos have expression similar to wild-type.
Specimen 6B KO-SetA right: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetA right 80% of Smad5 knockout embryos have expression similar to wild-type.
Specimen 6B KO-SetB left: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetB left 80% of Smad5 knockout embryos have expression similar to wild-type.
TS11: amnion ectoderm Present 6B KO-SetB left bottom , 6B KO-SetB left 20% of the mutants have ectopic expression in the amnion (shown).
Specimen 6B KO-SetB middle: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetB middle 80% of Smad5 knockout embryos have expression similar to wild-type.
TS11: amnion ectoderm Present 6B KO-SetB middle bottom , 6B KO-SetB middle 20% of the mutants have ectopic expression in the amnion (shown).
Specimen 6B KO-SetB right: embryonic day 7.5; Smad5tm1Zuk/Smad5tm1Zuk (more )
Note: Double labeled: red - Elf5; green - Pou5f1 (assay MGI:6510822).
Structure Level Pattern Image Note
TS11: chorion Present 6B KO-SetB right 80% of Smad5 knockout embryos have expression similar to wild-type.
TS11: amnion ectoderm Present 6B KO-SetB right , 6B KO-SetB right bottom 20% of the mutants have ectopic expression in the amnion (shown).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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