Antibody:	anti-calbindin D-28K
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted	5E	Thin but significant molecular layer and granule cell layers are detected with scattered Purkinje cells between these layers.
TS28: cerebellum molecular layer	Present	Regionally restricted	5E	Thin but significant molecular layer and granule cell layers are detected with scattered Purkinje cells between these layers.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Regionally restricted	5F	Authors report molecular layer is defective. Extensive distubance of Purkinje cell migration results in narrowing of molecular layer that consists of dendrites of Purkinje cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted		Identical defects in cerebellum also observed in p35-/- rl/+ mice.