Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:138770
Ten Berge D, et al., Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development. Development. 2008 Oct;135(19):3247-57
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6509999
|
|
Gene symbol:
|
Sox9
|
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
|
Antibody:
|
anti-Sox9
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
|
|
|
Results
|
Specimen
7B:
embryonic day 9.5; Axin2tm1Wbm/Axin2 +
(more  )
Note: 22 somites.
Specimen
7B:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
22 somites.
|
Specimen
7D:
embryonic day 9.5; Axin2tm1Wbm/Axin2 +
(more )
Note: 27 somites.
Specimen
7D:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
27 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
7D
|
At late E9.5 (27 somites), Sox9 is expressed in the chondrogenic population.
|
Specimen
7E:
embryonic day 11.5
(more )
Specimen
7E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7E
|
Expression was detected in the chondrogenic population.
|
Specimen
7F:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Mycntm1.1Psk/Mycntm1.1Psk
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7F
|
The Sox9 domain was reduced in the mutant.
|
Specimen
7I:
embryonic day 11.5
(more )
Specimen
7I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7I
|
Expression was detected in the chondrogenic population.
|
|
Analysis Tools
