Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:138770
Ten Berge D, et al., Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development. Development. 2008 Oct;135(19):3247-57
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:6509988
|
|
Gene symbol:
|
Axin2
|
|
Gene name:
|
axin 2
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
5A:
embryonic day 10.5; Axin2tm1Wbm/Axin2 +
(more  )
Specimen
5A:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5A
|
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
|
Specimen
5B:
embryonic day 11.5; Axin2tm1Wbm/Axin2 +
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5B
|
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
|
Specimen
5C:
embryonic day 11.5; Axin2tm1Wbm/Axin2 +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
5C
|
Expression was detected approximately 100 um layer of mesenchyme underneath the ectoderm in all regions of the limb bud. Expression co-localized with proliferation (BrdU staining). Chondrogenic differentiation (Sox9 immunostaining) was mutually exclusive with proliferation and reporter expression.
|
Specimen
7A:
embryonic day 9.5; Axin2tm1Wbm/Axin2 +
(more )
Note: 22 somites.
Specimen
7A:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
22 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Widespread |
7A
|
Expression was visible throughout the forelimb bud.
|
Specimen
7C:
embryonic day 9.5; Axin2tm1Wbm/Axin2 +
(more )
Note: 27 somites.
Specimen
7C:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
27 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
7C
|
Expression was no longer active in the center of the forelimb.
|
Specimen
S1B:
embryonic day 13.5; Axin2tm1Wbm/Axin2 +
(more )
Specimen
S1B:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Axin2tm1Wbm/Axin2+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools
