Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32994
Puliti A, et al., Neuronal defects in genotyped dominant megacolon (Dom) mouse embryos, a model for Hirschsprung disease. Neuroreport. 1996 Jan 31;7(2):489-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6509980
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
E11 control not shown:
embryonic day 11.0
(more  )
Specimen
E11 control not shown:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: intestine |
Present |
Regionally restricted |
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Authors report morphological variation in intestinal innervation in control embryos.
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Specimen
E13 control not shown:
embryonic day 13.0
(more )
Specimen
E13 control not shown:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: large intestine |
Present |
Regionally restricted |
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Normal embryos showed radial extension of neuroblasts into proximal colon.
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| TS21: cecum |
Present |
Regionally restricted |
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Authors report spread of neurons in cecum.
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Specimen
E13 mutant not shown:
embryonic day 13.0; Sox10Dom/Sox10 +
(more )
Specimen
E13 mutant not shown:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Sox10Dom/Sox10+
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: large intestine |
Present |
Regionally restricted |
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Mutant embryos show neuroblasts only along mesenteric border of large intestine.
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Specimen
2A:
embryonic day 14.0; Sox10Dom/Sox10 +
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Sox10Dom/Sox10+
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: large intestine |
Present |
Regionally restricted |
2A
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Authors report a variable defect of innervation in mutant embryos.
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Specimen
2B:
embryonic day 14.0; Sox10Dom/Sox10 +
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Sox10Dom/Sox10+
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: large intestine |
Present |
Regionally restricted |
2B
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Authors report a variable defect of innervation in mutant embryos.
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Specimen
2C:
embryonic day 14.0
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: large intestine |
Present |
Regionally restricted |
2C
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In normal embryos, distal large intestine is fully innervated.
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Analysis Tools