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Gene Expression Data
Assay Details
Assay
Reference: J:172683 Lodder-Gadaczek J, et al., N-Acetylaspartylglutamate Synthetase II Synthesizes N-Acetylaspartylglutamylglutamate. J Biol Chem. 2011 May 13;286(19):16693-706
Assay type: Northern blot
MGI Accession ID: MGI:6509474
Gene symbol: Rimkla
Gene name: ribosomal modification protein rimK-like family member A
Probe: pFLAG-NAAGS-II
Probe preparation: Antisense labelled with digoxigenin RNA
Assay notes: The authors state that some tissues had weak expression but that this was not visible in this scanned film and verified these data with quantitative RT-PCR (shown in Figure 8B).
Results
Image: 8A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
1 E12.5 TS20: head Present (a) 20 µg; total RNA C57BL/6 Not Specified
2 E12.5 TS20: trunk Present (a) 20 µg; total RNA C57BL/6 Not Specified Body of embryo without head.
3 P w 10 TS28: cerebellum Strong 20 µg; total RNA C57BL/6 Male
4 P w 10 TS28: brainstem Very strong 20 µg; total RNA C57BL/6 Male
5 P w 10 TS28: forebrain Strong 20 µg; total RNA C57BL/6 Male
6 P w 10 TS28: brain Strong 20 µg; total RNA C57BL/6 Male
7 P w 10 TS28: liver Trace 20 µg; total RNA C57BL/6 Male
8 P w 10 TS28: spleen Trace 20 µg; total RNA C57BL/6 Male
9 P w 10 TS28: metanephros Trace 20 µg; total RNA C57BL/6 Male
10 P w 10 TS28: adrenal gland Trace 20 µg; total RNA C57BL/6 Male
11 P w 10 TS28: testis Trace 20 µg; total RNA C57BL/6 Male
12 P w 10 TS28: lung Trace 20 µg; total RNA C57BL/6 Male
13 P w 10 TS28: thymus Trace 20 µg; total RNA C57BL/6 Male
14 P w 10 TS28: heart Trace 20 µg; total RNA C57BL/6 Male
15 P w 10 TS28: skeletal muscle Trace 20 µg; total RNA C57BL/6 Male
16 P w 10 TS28: eye Trace 20 µg; total RNA C57BL/6 Male
17 P w 10 TS28: spinal cord Very strong 5 µg; total RNA C57BL/6 Male
Notes:
(a) Present assigned by curator.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory