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Gene Expression Data
Assay Details
Assay
Reference: J:300004 Getachew D, et al., Morphologic changes in the cytoskeleton and adhesion apparatus during the conversion from pseudostratified single columnar to stratified squamous epithelium in the developing mouse esophagus. Congenit Anom (Kyoto). 2021 Jan;61(1):14-24
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6509452
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: anti-ZO-1 (# 61-7300)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
Results
Specimen 4a: embryonic day 11.5 (more )
Note: Embryo fixed in 4% formaldehyde/70% methanol.
Structure Level Pattern Image Note
TS19: esophagus epithelium Present 4a At this age expression was apically localized, indicating a pseudostratified single columnar epithelium.
Specimen 4b: embryonic day 13.5 (more )
Note: Embryo fixed in 4% formaldehyde/70% methanol.
Structure Level Pattern Image Note
TS21: esophagus epithelium Present 4b At this age expression appears dispersed/disturbed, suggesting a change in the shape/size and arrangement/orientation of the epithelial cells.
Specimen 4c: embryonic day 14.5 (more )
Note: Embryo fixed in 4% formaldehyde/70% methanol.
Structure Level Pattern Image Note
TS22: esophagus epithelium Present 4c At this age expression starts to reappear apically and in the intermediate cell layer.
Specimen 4d: embryonic day 15.5 (more )
Note: Embryo fixed in 4% formaldehyde/70% methanol.
Structure Level Pattern Image Note
TS23: esophagus epithelium Present 4d At this age expression is observed apically and subapically in an organized manner, indicating a two-layered squamous epithelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory