Gene Expression Data
Assay Details
Assay
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Reference:
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J:37194
Thomas P, et al., Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo. Curr Biol. 1996 Nov 1;6(11):1487-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6509302
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Gene symbol:
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Hesx1
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Gene name:
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homeobox gene expressed in ES cells
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Probe:
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Hesx1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1a:
embryonic day 6.5
(more )
Specimen
1a:
(close )
Genetic Background:
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(C57BL/10 x CBA)F1
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Age:
|
embryonic day 6.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo endoderm |
Present |
Regionally restricted |
1a
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Expressed in a small patch of cells (10-20 cells) in the anterior midline. This region was separated from the extraembryonic-embryonic junction by approximately five endoderm cell diameters.
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Specimen
1b:
embryonic day 6.5
(more )
Note: early-streak stage
Specimen
1b:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
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Age:
|
embryonic day 6.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
|
Note:
|
early-streak stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo endoderm |
Present |
Regionally restricted |
1b
|
Expression remained restricted to a small patch of endoderm cells during primitive streak elongation.
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Specimen
1c:
embryonic day 7.0
(more )
Note: late-streak stage
Specimen
1c:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
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Age:
|
embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Note:
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late-streak stage
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Structure |
Level |
Pattern |
Image |
Note |
TS10: neural ectoderm |
Weak |
Regionally restricted |
1c
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Expressed in the prospective neurectoderm immediately adjacent to the hybridizing endoderm domain.
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TS10: embryo endoderm |
Present |
Regionally restricted |
1c
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Expressed in the anterior endoderm.
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Specimen
1d:
embryonic day 7.5
(more )
Note: headfold stage
Specimen
1d:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
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Age:
|
embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
|
Note:
|
headfold stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: neural ectoderm |
Present |
Regionally restricted |
1d
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Expression in the anterior neurectoderm intensified and spread laterally during formation of the cranial neural folds to encompass the developing prosencephalon.
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Specimen
1e:
embryonic day 7.5
(more )
Note: neural plate stage
Specimen
1e:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
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Age:
|
embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Note:
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neural plate stage
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Structure |
Level |
Pattern |
Image |
Note |
TS11: neural ectoderm |
Present |
Regionally restricted |
1e
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Expression in the anterior neurectoderm intensified and spread laterally during formation of the cranial neural folds to encompass the developing prosencephalon.
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Specimen
1f:
embryonic day 7.5
(more )
Note: headfold stage
Specimen
1f:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
|
Age:
|
embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
|
Type:
|
whole mount
|
Note:
|
headfold stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: neural ectoderm |
Present |
Regionally restricted |
1f
|
Expression in the anterior neurectoderm intensified and spread laterally during formation of the cranial neural folds to encompass the developing prosencephalon.
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Specimen
1g:
embryonic day 6.5
(more )
Note: early-streak stage
Specimen
1g:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
|
Age:
|
embryonic day 6.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
|
early-streak stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo endoderm |
Present |
Regionally restricted |
1g
|
Expression remained restricted to a small patch of endoderm cells during primitive streak elongation.
|
Specimen
1i:
embryonic day 6.5
(more )
Note: Early-streak stage. Also stained for T (assay MGI:6509305).
Specimen
1i:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Early-streak stage. Also stained for T (assay MGI:6509305).
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo endoderm |
Present |
Regionally restricted |
1i
|
Expression remained restricted to a small patch of endoderm cells.
|
Specimen
1j:
embryonic day 7.0
(more )
Note: Midstreak stage. Also stained for T (assay MGI:6509305).
Specimen
1j:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Midstreak stage. Also stained for T (assay MGI:6509305).
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo endoderm |
Present |
Regionally restricted |
1j
|
Expression remained restricted to a small patch of endoderm cells.
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Specimen
1k:
embryonic day 8.0
(more )
Note: early somite-stage
Specimen
1k:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
|
Age:
|
embryonic day 8.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Note:
|
early somite-stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: neural ectoderm |
Present |
Regionally restricted |
1k
|
Expression in the anterior neurectoderm intensified and spread laterally during formation of the cranial neural folds to encompass the developing prosencephalon.
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TS12: future prosencephalon |
Present |
|
1k
|
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Specimen
1l:
embryonic day 8.0
(more )
Note: early somite-stage
Specimen
1l:
(close )
Genetic Background:
|
(C57BL/10 x CBA)F1
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Age:
|
embryonic day 8.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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early somite-stage
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Specimen
1m:
embryonic day 9.5
(more )
Specimen
1m:
(close )
Genetic Background:
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(C57BL/10 x CBA)F1
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Specimen
1n:
embryonic day 11.5
(more )
Specimen
1n:
(close )
Genetic Background:
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(C57BL/10 x CBA)F1
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Specimen
1o:
embryonic day 11.5
(more )
Specimen
1o:
(close )
Genetic Background:
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(C57BL/10 x CBA)F1
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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