Reporter:	lacZ
	Direct Detection
Assay notes:	Specimens were fixed in 0.2% glutaraldehyde and post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS20: arterial system endothelium	Strong	Regionally restricted	2B	Expression is in the endothelium of large arteries and in major arteries branching out from the aortic trunk.
TS20: internal carotid artery	Strong	Regionally restricted	2B	Expression is in the endothelium
TS20: umbilical artery	Strong	Regionally restricted	2B	Expression is in the endothelium
TS20: vertebral artery	Strong	Regionally restricted	2B	Expression is in the endothelium
TS20: dorsal aorta	Present	Regionally restricted	2B	Expression is in the aortic endothelium in the thoracic and in the abdominal region.

Structure	Level	Pattern	Image	Note
TS20: carotid artery	Present	Regionally restricted	3A	Expression is in the carotid arteries and their branches in the basal brain.
TS20: heart left ventricle	Present		3A
TS20: heart right ventricle	Present		3A
TS20: vertebral artery	Present		3A

Structure	Level	Pattern	Image	Note
TS20: carotid artery	Present	Regionally restricted	3B	Expression is in the branch point of the internal and external carotid artery at the base of the brain.
TS20: vertebral artery	Present		3B

Structure	Level	Pattern	Image	Note
TS20: vascular element	Present	Regionally restricted	3C	Expression is in the circle of Willis.
TS20: vertebral artery	Present		3C

Structure	Level	Pattern	Image	Note
TS20: midbrain vascular element	Strong	Regionally restricted	3D	Expression is in the superior sagittal dural venous sinus in the midline of the roof of the midbrain.
TS20: vascular element	Strong	Regionally restricted	3D	Expression is in the superior sagittal dural venous sinus in the midline of the roof of the midbrain.

Structure	Level	Pattern	Image	Note
TS20: arterial system endothelium	Very strong	Regionally restricted	3E	Expression is in the endothelium of the umbilical arteries.
TS20: umbilical artery embryonic part	Very strong	Regionally restricted	3E	Expression is in the endothelium of the umbilical arteries.
TS20: artery	Present	Regionally restricted	3E	Expression is in intercostal arteries branching from the descending aorta and is weaker in the more distal branches.

Structure	Level	Pattern	Image	Note
TS20: arterial system endothelium	Very strong	Regionally restricted	3F	Expression is in the endothelium of the umbilical arteries.
TS20: umbilical artery embryonic part	Very strong	Regionally restricted	3F	Expression is in the endothelium of the umbilical arteries.
TS20: heart	Present		3F
TS20: umbilical vein	Absent		3F

Structure	Level	Pattern	Image	Note
TS20: arterial system endothelium	Very strong	Regionally restricted	3G	Expression is in the umbilical artery entering the placenta and the chorionic plate.
TS20: common umbilical artery	Very strong	Regionally restricted	3G	Expression is in the umbilical artery entering the placenta and the chorionic plate.
TS20: umbilical vein	Absent		3G

Structure	Level	Pattern	Image	Note
TS20: extraembryonic arterial system	Strong	Regionally restricted	3H	Expression is in the largest amniotic arteries.
TS20: umbilical vein	Absent		3H

Structure	Level	Pattern	Image	Note
TS23: endocardial lining	Weak		4A
TS23: ventricle endocardial lining	Weak		4A
TS23: blood vessel endothelium	Weak	Regionally restricted	4A	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.
TS23: inferior vena cava	Weak	Regionally restricted	4A	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.
TS23: superior vena cava	Weak	Regionally restricted	4A	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.

Structure	Level	Pattern	Image	Note
TS23: blood vessel endothelium	Weak	Regionally restricted	4B	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.
TS23: inferior vena cava	Weak	Regionally restricted	4B	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.
TS23: superior vena cava	Weak	Regionally restricted	4B	Expression is in the endothelium of the superior and inferior venae cavae close to the sites where they enter the heart.
TS23: artery endothelium	Present	Regionally restricted	4B	Expression is in the endothelium in the major arteries around the heart and also in the peripheral arteries of the body wall.
TS23: pulmonary artery	Present	Regionally restricted	4B	Expression is in the endothelium.
TS23: artery	Present	Regionally restricted	4B	Expression is in the endothelium of major arteries around the heart and in the peripheral arteries of the body wall.

Structure	Level	Pattern	Image	Note
TS23: arterial blood vessel	Strong	Regionally restricted	4C	Expression is in the endothelium of major arteries around the heart and in the peripheral arteries of the body wall.
TS23: artery endothelium	Strong	Regionally restricted	4C	Expression is in the femoral and caudal artery.
TS23: femoral vein	Absent		4C

Structure	Level	Pattern	Image	Note
TS23: artery endothelium	Present	Regionally restricted	4D	Expression is in the radial artery endothelium.

Structure	Level	Pattern	Image	Note
TS23: umbilical artery embryonic part	Present	Regionally restricted	4E	Expression is in the endothelium.
TS23: umbilical vein embryonic part	Absent		4E

Structure	Level	Pattern	Image	Note
TS23: abdominal wall	Present	Regionally restricted	4F	Expression is in blood vessels in the abdominal wall.
TS23: arterial system endothelium	Present	Regionally restricted	4F	Expression is in blood vessels in the abdominal wall.
TS23: abdomen blood vessel	Absent		4F	Absent in blood vessels in the abdominal organs.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present	Regionally restricted	6B	Expression is in brain microvasculature and major vessels, absent in the smallest arterioles.
TS28: brain blood vessel	Present	Regionally restricted	6B	Expression is in brain microvasculature and major vessels, absent in the smallest arterioles.
TS28: capillary	Absent		6B

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present	Regionally restricted	6C	Expression is in brain microvasculature and major vessels, absent in the smallest arterioles.
TS28: brain blood vessel	Present	Regionally restricted	6C	Expression is in brain microvasculature and major vessels, absent in the smallest arterioles.
TS28: capillary	Absent		6C

Structure	Level	Pattern	Image	Note
TS28: inferior vena cava	Weak		6D
TS28: superior vena cava	Weak		6D

Structure	Level	Pattern	Image	Note
TS28: coronary artery	Strong		6E
TS28: heart atrium	Strong	Regionally restricted	6E	Expression is in the endothelium of the auricles.
TS28: heart endothelium	Strong	Regionally restricted	6E	Expression is in the endothelium of the auricles.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present	Regionally restricted	6F	Expression is in the endothelium of the coronary artery.
TS28: coronary artery	Present	Regionally restricted	6F	Expression is in the endothelium of the coronary artery.
TS28: coronary sinus	Absent		6F

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present	Spotted	7A
TS28: hassall's corpuscle	Present		7A

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present	Spotted	7B inset , 7B
TS28: blood vessel	Present	Regionally restricted	7B inset , 7B	Expression is in blood vessels in the medulla of the thymus.
TS28: hassall's corpuscle	Present	Regionally restricted	7B inset , 7B	Expression is in epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Present	Patchy	7C

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Present	Regionally restricted	7D , 7D inset	Expression is in certain basal cells in the mucosa and becomes weaker when the epithelial cells keratinize and move upward.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Strong	Regionally restricted	8B	Expression is uniform in arteries on the surface of the brain cortex.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present	Regionally restricted	8C	Expression is in the arterial endothelium in arteries on the surface of the brain cortex in a mosaiclike pattern.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Strong	Regionally restricted	8E	Expression is uniform in arteries on the surface of the brain cortex.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present	Regionally restricted	8F	Expression is in the arterial endothelium in arteries on the surface of the brain cortex in a mosaiclike pattern.

Structure	Level	Pattern	Image	Note
TS28: aorta endothelium	Present		10A

Structure	Level	Pattern	Image	Note
TS20: pulmonary trunk	Present	Regionally restricted		Expression is in arteries branching from the pulmonary trunk and is weaker in the more distal branches.

Structure	Level	Pattern	Image	Note
TS23: midbrain vascular element	Strong	Regionally restricted		Expression is in the superior sagittal dural venous sinus in the midline of the roof of the midbrain.
TS23: vascular element	Strong	Regionally restricted		Expression is in the superior sagittal dural venous sinus in the midline of the roof of the midbrain.