Gene Expression Data
Assay Details
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Assay
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Reference:
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J:151992
Vanhoutteghem A, et al., Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14432-7
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6509039
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Gene symbol:
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Bnc2
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Gene name:
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basonuclin zinc finger protein 2
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Results
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Specimen
4A:
embryonic day 14.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more  )
Note: Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4A:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf |
Moderate |
Regionally restricted |
4A
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Expression was detected in palatal processes.
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| TS22: head |
Moderate |
Regionally restricted |
4A
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Staining was virtually confined to the anterior part of the palatal processes. Little staining was detected in the rest of the head.
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Specimen
4B:
embryonic day 14.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Anterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Anterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf |
Moderate |
Regionally restricted |
4B
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Expression was detected in the anterior region of the palatal processes.
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Specimen
4C:
embryonic day 14.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Posterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Posterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf |
Absent |
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4C
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The posterior region of the palatal processes was not stained.
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Specimen
4D:
embryonic day 16.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: secondary palate |
Strong |
Regionally restricted |
4D
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Stronger staining was present along the entire anteroposterior axis of the palate.
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| TS24: head |
Strong |
Regionally restricted |
4D
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Stronger staining was noted in sheaths of cells around the brain.
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| TS24: molar |
Strong |
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4D
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| TS24: nasal cartilage |
Strong |
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4D
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| TS24: tongue |
Strong |
Regionally restricted |
4D
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Stronger staining was noted at the base and the periphery of the tongue.
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Specimen
4E:
embryonic day 16.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Anterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4E:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Anterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: secondary palate |
Strong |
Regionally restricted |
4E
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Stronger staining was present along the entire anteroposterior axis of the palate.
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| TS24: tongue |
Strong |
Regionally restricted |
4E
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Stronger staining was noted at the periphery of the tongue.
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Specimen
4F:
embryonic day 16.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Posterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Posterior region of palatal processes. Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: secondary palate |
Strong |
Regionally restricted |
4F
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Stronger staining was present along the entire anteroposterior axis of the palate.
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| TS24: tongue |
Strong |
Regionally restricted |
4F
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Stronger staining was noted at the periphery of the tongue.
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Specimen
4G/H:
embryonic day 16.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
Specimen
4G/H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - pan-keratin. Fix: methanol/acetone
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Specimen
5G:
embryonic day 14.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
Specimen
5G:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Present |
Regionally restricted |
5G
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The frequency of phosphohistone staining was similar in areas expressing and not expressing Beta-gal.
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Specimen
5H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Present |
Regionally restricted |
5H
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The frequency of phosphohistone staining was predominantly reduced in areas with high expression of beta-gal.
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Specimen
5I:
embryonic day 16.5; Bnc2Gt(pU21)18Imeg/Bnc2 +
(more )
Note: Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
Specimen
5I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
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Specimen
5J:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bnc2; green - phosphohistone H3. Fix: methanol/acetone
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: nasal septum |
Present |
Regionally restricted |
5J
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The scarcity of mitoses in beta-gal-expressing regions was observed in the nasal septum.
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