Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz
Transgene Detail
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| Symbol: |
Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz |
| Name: |
transgene insertion 2, Noboru Mizushima |
| MGI ID: |
MGI:6508991 |
| Synonyms: |
Tg(CAG-GFP/LC3/RFP/LC3*deltaG)2Nmz |
| Transgene: |
Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz Location: unknown
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| Alliance: |
Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz page
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: This transgene expresses GFP-LC3-RFP-LC3DeltaG, a fluorescent probe to evaluate autophagic flux, under the control of a CAG promoter. The fusion protein is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3DeltaG. GFP-LC3 is degraded by autophagy, while RFP-LC3DeltaG remains in the cytosol, serving as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. In this line, high expression was observed only in the skeletal muscle.
(J:252211)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:252211 Kaizuka T, et al., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 |
| All: |
2 reference(s) |
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Analysis Tools
