About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:54582 Iacopetti P, et al., Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4639-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6508687
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-beta III tubulin
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 2A/C: embryonic day 10.0 (more )
Note: Data was quantified in Fig 3. Double labeled: brown - Tubb3; purple - Btg2 (assay MGI:6508686).
Structure Level Pattern Image Note
TS16: diencephalon Present Single cells 2C , 2A Expressed in only a few cells in the neuroepithelium in regions where neurogenesis was just starting.
Specimen 2B/D: embryonic day 10.0 (more )
Note: Data was quantified in Fig 3. Double labeled: brown - Tubb3; purple - Btg2 (assay MGI:6508686).
Structure Level Pattern Image Note
TS16: future hindbrain Present 2D , 2B Expressed in many more cells in the neuroepithelium.
Specimen not shown: embryonic day 10.0 (more )
Note: Data was quantified in Fig 3. Also stained for Btg2 (assay MGI:6508686).
Structure Level Pattern Image Note
TS16: future midbrain Present Increased expression compared with the diencephalon.
Specimen 6C: embryonic day 10.0 (more )
Note: Double labeled: brown - Tubb3; purple - Btg2 (assay MGI:6508686).
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 6C Expressed in newborn neurons.
Specimen 6D: embryonic day 10.0 (more )
Note: Double labeled: brown - Tubb3; purple - Btg2 (assay MGI:6508686).
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 6D Expressed in newborn neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory