Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Lgr5tm3.1(cre/ERT2)Niba
Name:	leucine rich repeat containing G protein coupled receptor 5; targeted mutation 3.1, Nick Barker
MGI ID:	MGI:6507189
Synonyms:	Lgr5-2A-creERT2
Gene:	Lgr5  Location: Chr10:115286219-115423685 bp, - strand  Genetic Position: Chr10, 63.51 cM
Alliance:	Lgr5tm3.1(cre/ERT2)Niba page

Germline Transmission:	Earliest citation of germline transmission: J:292580
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Lgr5tm3.1(cre/ERT2)Niba expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Lgr5 (MGI:1341817)   Mutation details: Sequence for the 2A self-cleaving peptide, a cre-ERT2 fusion gene and a loxP site flanked neomycin resistance gene cassette were inserted immediately before the stop codon. The neo cassette was removed through subsequent cre-mediated recombination. This allele expresses the endogenous peptide and the inserted cre-ERT2 fusion peptide. (J:247209, J:292580)

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lgr5 Mutation:	57 strains or lines available

Original:	J:247209 Leushacke M, et al., Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach. Nat Cell Biol. 2017 Jul;19(7):774-786
All:	5 reference(s)