Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)250Nagy
Name:	transgenic transposon concatemer 250, Andras Nagy
MGI ID:	MGI:6507139
Synonyms:	OKMSCh250
Transgene:	TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)250Nagy  Location: unknown  Genetic Position: Chr14, Syntenic
Alliance:	TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)250Nagy page

Strain of Origin:

129 and ICR

Transgene Type:    Transgenic (Inserted expressed sequence, Reporter, Transposon concatemer) Mutation:    Insertion   TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)250Nagy expresses 4 genes Transgene expresses: Organism Expressed Gene Note mouse Klf4 (MGI:1342287) mouse Myc (MGI:97250) mouse Pou5f1 (MGI:101893) mouse Sox2 (MGI:98364)   Mutation details: The transgene consists of a piggy-BAC transposon delivery construct: PB:TetO-OKMS-mCherry. PB:TetO-OKMS-mCherry contains a tetracycline responsive element (TRE; tetO), the polycistronic expression cassette OKMS, an IRES, the mCherry gene and polyA signal. The second generation TRE (SG-TRE) used here is tetO2 - containing a shortened CMV minimal promoter with eight copies of 19 bp tet operator sequences positioned in an optimized manner upstream of the TATA box. The OKMS cassette contains four mouse reprogramming genes - Oct4 (Pou5f1), Klf4, c-Myc (Myc) and Sox2 - each separated by viral 2A oligopeptides that mediate ribosomal skipping (F2A, E2A and F2A, respectively). Founder line 250 harbors a single transgene insertion on chromosome 14:97,485,545-97,485,549[mm9]). (J:101977)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available      Cell Lines: 0 lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)