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Gene Expression Data
Assay Details
Assay
Reference: J:296649 Lindstrom NO, et al., Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning. J Am Soc Nephrol. 2018 Mar;29(3):825-840
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6505980
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-LEF1 (N-17; sc-8591)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors used two Lef1 antibodies; it is possible that MGI:5629674 was used to obtain some or all of these data.
Results
Specimen 1B: embryonic day 15.5 (more )
Note: Triple labeled: green - Lef1; red - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS23: renal vesicle Present 1B right , 1B left Expression of this gene is one of the earliest markers of the initiation of nephrogenesis.
Specimen 2C': embryonic day 15.5 (more )
Note: Triple labeled: green - Lef1; red - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS23: comma-shaped body Present 2C'
TS23: s-shaped body Present 2C'
Specimen 4A - RV: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: distal renal vesicle Present 4A LEF1 - RV
Specimen 4A - SSB: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: distal segment of s-shaped body Present 4A LEF1 - SSB
TS23: medial segment of s-shaped body Present 4A LEF1 - SSB
TS23: proximal segment of s-shaped body Present 4A LEF1 - SSB
Specimen S2 upper: postnatal day 2 (more )
Note: Triple labeled: red - Lef1; green - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS27: comma-shaped body Present S2_upper
TS27: renal vesicle Present S2_upper
TS27: s-shaped body Present S2_upper
Specimen S2 lower: postnatal day 2 (more )
Note: Triple labeled: red - Lef1; green - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS27: early nephron Present S2_lower
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory