About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:296649 Lindstrom NO, et al., Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning. J Am Soc Nephrol. 2018 Mar;29(3):825-840
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6505980
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-LEF1 (N-17; sc-8591)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors used two Lef1 antibodies; it is possible that MGI:5629674 was used to obtain some or all of these data.
Results
Specimen 1B: embryonic day 15.5 (more )
Note: Triple labeled: green - Lef1; red - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS23: renal vesicle Present 1B right , 1B left Expression of this gene is one of the earliest markers of the initiation of nephrogenesis.
Specimen 2C': embryonic day 15.5 (more )
Note: Triple labeled: green - Lef1; red - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS23: comma-shaped body Present 2C'
TS23: s-shaped body Present 2C'
Specimen 4A - RV: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: distal renal vesicle Present 4A LEF1 - RV
Specimen 4A - SSB: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: distal segment of s-shaped body Present 4A LEF1 - SSB
TS23: medial segment of s-shaped body Present 4A LEF1 - SSB
TS23: proximal segment of s-shaped body Present 4A LEF1 - SSB
Specimen S2 upper: postnatal day 2 (more )
Note: Triple labeled: red - Lef1; green - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS27: comma-shaped body Present S2_upper
TS27: renal vesicle Present S2_upper
TS27: s-shaped body Present S2_upper
Specimen S2 lower: postnatal day 2 (more )
Note: Triple labeled: red - Lef1; green - Six2 (assay MGI:6505986); blue - Krt8 (assay MGI:6505962)
Structure Level Pattern Image Note
TS27: early nephron Present S2_lower
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory