Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80961
Jitpukdeebodintra S, et al., Developmental patterning of the circumvallate papilla. Int J Dev Biol. 2002 Aug;46(5):755-63
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6505790
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Gene symbol:
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Cdh1
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Gene name:
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cadherin 1
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Antibody:
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anti-E-cadherin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4A:
embryonic day 12.0
(more )
Specimen
4A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
4A
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Expression is intense in all cell layers at the anterior end of the circumvallate papilla placode and is diffuse in the epithelial cells at the tip of the placode.
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Specimen
4B:
embryonic day 12.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
4B
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Expression is most intense in the epithelial cells in the superficial and the basal cell layers and is very weak in the epithelial cells in the middle layer of the placode.
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Specimen
4C:
embryonic day 13.0
(more )
Specimen
4C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Carnoy's Fixative
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Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
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4C
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Expression is in the superficial epithelial cell layer and in the epithelial cells at the middle of the ring structure of the circumvallate papilla placode and is very weak in the epithelial cells positioned between these two layers as well as among the epithelial cells near the basement membrane.
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Specimen
4D:
embryonic day 13.0
(more )
Specimen
4D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
Regionally restricted |
4D
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Expression is in the epithelial cells of the ring area at the site where the nerve will penetrate.
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Specimen
4E:
embryonic day 13.0
(more )
Specimen
4E:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
Regionally restricted |
4E
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Expression is intense in the superficial epithelial cell layer in the dome area and is weaker in the epithelial cells near the basement membrane.
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Specimen
4F:
embryonic day 13.0
(more )
Specimen
4F:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
Regionally restricted |
4F
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Expression is most intense in the superficial epithelial cell layer at the anterior end is absent in the epithelial cell mass closest to the basement membrane.
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Specimen
not shown E12 Posterior:
embryonic day 12.0
(more )
Specimen
not shown E12 Posterior:
(close )
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Genetic Background:
|
Swiss Webster
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|
Age:
|
embryonic day 12.0
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|
Sex:
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Not Specified
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Type:
|
section
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|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
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At the posterior end of the of the circumvallate papilla placode expression is intense in the epithelial cells from the basement membrane toward the epithelial cells in the superficial layer.
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Specimen
not shown E12.5 Posterior:
embryonic day 12.5
(more )
Specimen
not shown E12.5 Posterior:
(close )
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Genetic Background:
|
Swiss Webster
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|
Age:
|
embryonic day 12.5
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|
Sex:
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Not Specified
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Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
|
At the posterior end of the of the circumvallate papilla placode expression is intense in the epithelial cells from the basement membrane toward the epithelial cells in the superficial layer.
|
Specimen
not shown E12 Middle:
embryonic day 12.0
(more )
Specimen
not shown E12 Middle:
(close )
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Genetic Background:
|
Swiss Webster
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|
Age:
|
embryonic day 12.0
|
|
Sex:
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Not Specified
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Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
|
In the middle portion of the circumvallate papilla placode, expression is most intense in the superficial epithelial cell layer and in the epithelial cells along the basement membrane.
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Specimen
not shown E12.5 Middle:
embryonic day 12.5
(more )
Specimen
not shown E12.5 Middle:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 12.5
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|
Sex:
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Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Very strong |
Regionally restricted |
|
In the middle portion of the circumvallate papilla placode, expression is most intense in the superficial epithelial cell layer and in the epithelial cells along the basement membrane.
|
Specimen
not shown E13 Posterior:
embryonic day 13.0
(more )
Specimen
not shown E13 Posterior:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.0
|
|
Sex:
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Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
Regionally restricted |
|
At the posterior end of the circumvallate papilla placode, expression is decreased gradually from the superficial cell layer to the basal cell layer: being most intense in the epithelial cells along the superficial layer and less intense in the epithelial cells along the basement membrane.
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Specimen
not shown E13.5 Posterior:
embryonic day 13.5
(more )
Specimen
not shown E13.5 Posterior:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
Carnoy's Fixative
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tongue papillary placode |
Very strong |
Regionally restricted |
|
At the posterior end of the circumvallate papilla placode, expression is decreased gradually from the superficial cell layer to the basal cell layer: being most intense in the epithelial cells along the superficial layer and less intense in the epithelial cells along the basement membrane.
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