Antibody:	anti-ACP2 (4B5) sc-100344
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	1Aa , 1A	Expression is in a stream of cells.
TS16: future hindbrain	Present	Regionally restricted	1A , 1Bb , 1Cc , 1B , 1C	Expression is in scattered cells within the dorsal metencephalon.
TS16: head mesenchyme	Present	Regionally restricted	1A	Expression is in the mesodermal tissue that extends to the neuroepithelium of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS27: midbrain	Strong	Regionally restricted	1D	Expression is caudal and is absent in the rostral midbrain.
TS27: 4th ventricle choroid plexus	Present		1D
TS27: cerebellum	Present		1D
TS27: cerebral cortex	Absent		1D
TS27: diencephalon	Absent		1D
TS27: medulla oblongata	Absent		1D
TS27: pons	Absent		1D
TS27: thalamus	Absent		1D

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present	Diffuse	1E , 1Ee

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Diffuse	1F

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	1G	Expression is in localized in Purkinje cell somata in the posterior lobe vermis and hemispheres, with weak expression observed in the anterior cerebellum.
TS28: cerebellum posterior vermis	Present	Regionally restricted	1G	Expression is in localized in Purkinje cell somata in the posterior lobe vermis and hemispheres, with weak expression observed in the anterior cerebellum.
TS28: cerebellum anterior vermis	Weak		1G

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	1H	Expression is in localized in Purkinje cell somata and extends into the dendrites.
TS28: cerebellum anterior vermis	Present	Regionally restricted	1H	Expression is in localized in Purkinje cell somata and extends into the dendrites.
TS28: cerebellum posterior vermis	Present	Regionally restricted	1H	Expression is in localized in Purkinje cell somata and extends into the dendrites.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Regionally restricted	1I	Expression is in Purkinje cell somata and dendrites in a pattern of parasagittal stripes.
TS28: cerebellum posterior lobe	Strong	Regionally restricted	1I	Expression is in Purkinje cell somata and dendrites in a pattern of parasagittal stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		2A	Expression is in localized in Purkinje cell somata.
TS28: cerebellum molecular layer	Strong	Regionally restricted	2A	Expression is in the Purkinje cell dendrites located in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2B	Expression is in the dendrites and in the Purkinje cell body, excluding the nucleus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2C	Immunoreactivity in the Purkinje cell somata vary from strong to weak or absent.
TS28: cerebellum molecular layer	Present	Regionally restricted	2C	There is differential staining of the Purkinje cell dendritic arbors in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2D	Expression is in an array of parasagittal stripes, both in the Purkinje cell layer and the molecuar layer.
TS28: cerebellum molecular layer	Present	Regionally restricted	2D	Expression is in an array of parasagittal stripes, both in the Purkinje cell layer and the molecuar layer.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	2D	Expression is in an array of parasagittal stripes, both in the Purkinje cell layer and the molecuar layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum anterior vermis	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum vermis lobule I	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum vermis lobule II	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum vermis lobule III	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum vermis lobule IV	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.
TS28: cerebellum vermis lobule V	Present	Regionally restricted	4A	Expression is heterogeneous in an array of narrow parasagittal stripes that are symmetrical about the midline.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule III	Present	Regionally restricted	4B	Expression is heterogeneous in an array of narrow parasagittal stripes .
TS28: cerebellum vermis lobule IV	Present	Regionally restricted	4B	Expression is heterogeneous in an array of narrow parasagittal stripes .
TS28: cerebellum vermis lobule V	Present	Regionally restricted	4B	Expression is heterogeneous in an array of narrow parasagittal stripes .
TS28: cerebellum vermis lobule X	Present	Uniform	4B
TS28: cerebellum Purkinje cell layer	Present		4B

Structure	Level	Pattern	Image	Note
TS28: cerebellum posterior vermis	Present	Uniform	4C
TS28: cerebellum vermis lobule VI	Present	Uniform	4C
TS28: cerebellum vermis lobule VII	Present	Uniform	4C
TS28: ansiform lobule crus II	Present	Regionally restricted	4C	Expression is in an array of wide parasagittal stripes in the vermis and continues laterally to the cerebellar hemispheres, crus II and paramedian.
TS28: cerebellum vermis lobule IX	Present	Regionally restricted	4C	Expression is in an array of wide parasagittal stripes in the vermis and continues laterally to the cerebellar hemispheres, crus II and paramedian.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	4C	Expression is in an array of wide parasagittal stripes in the vermis and continues laterally to the cerebellar hemispheres, crus II and paramedian.
TS28: paramedian lobule	Present	Regionally restricted	4C	Expression is in an array of wide parasagittal stripes in the vermis and continues laterally to the cerebellar hemispheres, crus II and paramedian.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5A	Expression is in a narrow stripe at the midline (P1+).
TS28: cerebellum vermis lobule I	Present	Regionally restricted	5A	Expression is in a narrow stripe at the midline (P1+).

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5C	Expression is in an array of five narrow parasagittal stripes.
TS28: cerebellum vermis lobule II	Present	Regionally restricted	5C	Expression is in an array of five narrow parasagittal stripes.
TS28: cerebellum vermis lobule III	Present	Regionally restricted	5C	Expression is in an array of five narrow parasagittal stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5E	Expression is in wide stripes with the P1+ (1+) stripe at the midline and P2+ (2+) stripes on either side.
TS28: cerebellum vermis lobule V	Present	Regionally restricted	5E	Expression is in wide stripes with the P1+ (1+) stripe at the midline and P2+ (2+) stripes on either side.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Uniform	5G
TS28: cerebellum vermis lobule VI	Present	Uniform	5G
TS28: cerebellum vermis lobule VII	Present	Uniform	5G

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5I	Expression is in parasagittal stripes.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	5I	Expression is in parasagittal stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5K	Expression is uniform in an array of wide parasagittal stripes.
TS28: cerebellum vermis lobule IX	Present	Regionally restricted	5K	Expression is uniform in an array of wide parasagittal stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Uniform	5M
TS28: cerebellum vermis lobule X	Present	Uniform	5M

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6A	Expression is in an array of stripes including the P4+ (4), P5+ (5), and P6+ (6) stripes.
TS28: lobule simplex	Present	Regionally restricted	6A	Expression is in an array of stripes including the P4+ (4), P5+ (5), and P6+ (6) stripes.

Structure	Level	Pattern	Image	Note
TS28: ansiform lobule	Present	Regionally restricted	6C	Expression is in an array of stripes including the P4b+ (4b), P5+ with subdivision a and b (5a, 5b), P6+ (6), and P7+ (7) stripes.
TS28: ansiform lobule crus II	Present	Regionally restricted	6C	Expression is in an array of stripes including the P4b+ (4b), P5+ with subdivision a and b (5a, 5b), P6+ (6), and P7+ (7) stripes.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6C	Expression is in an array of stripes including the P4b+ (4b), P5+ with subdivision a and b (5a, 5b), P6+ (6), and P7+ (7) stripes.
TS28: cerebellum vermis lobule VII	Present	Regionally restricted	6C	Expression is in an array of stripes including the P4b+ (4b), P5+ with subdivision a and b (5a, 5b), P6+ (6), and P7+ (7) stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6E
TS28: flocculus	Present	Regionally restricted	6E
TS28: paraflocculus	Present	Regionally restricted	6E