Gene Expression Data
Assay Details
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Assay
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Reference:
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J:122815
Giordani J, et al., Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11310-11315
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6504946
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe28
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
E10.5 Six1-/- not shown:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Ag:
embryonic day 11.5
(more  )
Specimen
1Ag:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Ai:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Ak:
embryonic day 11.5; Six1tm1Mair/Six1 +
(more )
Specimen
1Ak:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Am:
embryonic day 11.5; Six1tm1Mair/Six1 +
(more )
Specimen
1Am:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Ao:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Aq:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Trace |
Single cells |
1Aq
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Expression barely detectable. Few expressing cells.
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Specimen
1Ca:
embryonic day 12.5
(more )
Specimen
1Ca:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1Cc:
embryonic day 12.5
(more )
Specimen
1Cc:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
1Cc
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Expression in dorsal region,.
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Specimen
1Ce:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
1Ce
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Expression restricted to posterior domain.
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Specimen
1Cg:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Weak |
Regionally restricted |
1Cg
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Reduced expression in dorsal part.
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Specimen
6(I)a:
embryonic day 11.5
(more )
Specimen
6(I)a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(I)c:
embryonic day 11.5; Six1tm1Mair/Six1 +
(more )
Specimen
6(I)c:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(I)e:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(II) Myf5 PHL wt:
embryonic day 11.5
(more )
Specimen
6(II) Myf5 PHL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(II) Myf5 PHL Six1-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(II) Myf5 PHL Six1-/-Six4-/+:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(II) Myf5 FL wt:
embryonic day 11.5
(more )
Specimen
6(II) Myf5 FL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(II) Myf5 FL Six1-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 FL wt:
embryonic day 12.5
(more )
Specimen
6(III) Myf5 FL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 FL Six1-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 PHL wt:
embryonic day 12.5
(more )
Specimen
6(III) Myf5 PHL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 PHL Six1-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 DHL wt:
embryonic day 12.5
(more )
Specimen
6(III) Myf5 DHL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6(III) Myf5 DHL Six1-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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