Myf5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122815 Giordani J, et al., Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11310-11315
Assay type:	RNA in situ
MGI Accession ID:	MGI:6504946
Gene symbol:	Myf5
Gene name:	myogenic factor 5

Probe:	Myf5 probe28
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen E10.5 Six1-/- not shown: embryonic day 10.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

1Ag ©

Specimen 1Ag: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		1Ag

1Ai ©

Specimen 1Ai: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		1Ai

1Ak ©

Specimen 1Ak: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1Ak

1Am ©

Specimen 1Am: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	1Am	Expression reduced.

1Ao ©

Specimen 1Ao: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1Ao

1Aq ©

Specimen 1Aq: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Trace	Single cells	1Aq	Expression barely detectable. Few expressing cells.

1Ca ©

Specimen 1Ca: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		1Ca

1Cc ©

Specimen 1Cc: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	1Cc	Expression in dorsal region,.

1Ce ©

Specimen 1Ce: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	1Ce	Expression restricted to posterior domain.

1Cg ©

Specimen 1Cg: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Weak	Regionally restricted	1Cg	Reduced expression in dorsal part.

6(I) a ©

Specimen 6(I)a: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(I) a
TS19: hindlimb bud	Present		6(I) a

6(I) c ©

Specimen 6(I)c: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(I) c
TS19: hindlimb bud	Weak	Regionally restricted	6(I) c	Expression severely reduced,

6(I) e ©

Specimen 6(I)e: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(I) e
TS19: hindlimb bud	Present		6(I) e

6(II) Myf5 PHL wt ©

Specimen 6(II) Myf5 PHL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6(II) Myf5 PHL wt

6(II) Myf5 PHL Six1-/- ©

Specimen 6(II) Myf5 PHL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	6(II) Myf5 PHL Six1-/-	Weak expressin in ventral aspect.

6(II) Myf5 PHL Six1-/-Six4-/+ ©

Specimen 6(II) Myf5 PHL Six1-/-Six4-/+: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Trace		6(II) Myf5 PHL Six1-/-Six4-/+	Expression barely detectable.

Specimen 6(II) Myf5 FL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(II) Myf5 FL wt

Specimen 6(II) Myf5 FL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(II) Myf5 FL Six1-/-	Expression absent in Six1-/- FL.

Specimen 6(III) Myf5 FL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		6(III) Myf5 FL wt

Specimen 6(III) Myf5 FL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Trace	Regionally restricted	6(III) Myf5 FL Six1-/-	Most expression lost except in posterior aspect.

Specimen 6(III) Myf5 PHL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present		6(III) Myf5 PHL wt

Specimen 6(III) Myf5 PHL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 PHL Six1-/-	Expression detected dorsally.

Specimen 6(III) Myf5 DHL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 DHL wt	Expression detectable at 500 mm from tip of limb.

Specimen 6(III) Myf5 DHL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 DHL Six1-/-	Expression detectable at 900 mm from tip of limb.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 01/20/2026 MGI 6.24