Myf5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122815 Giordani J, et al., Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11310-11315
Assay type:	RNA in situ
MGI Accession ID:	MGI:6504946
Gene symbol:	Myf5
Gene name:	myogenic factor 5

Probe:	Myf5 probe28
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen E10.5 Six1-/- not shown: embryonic day 10.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

1Ag ©

Specimen 1Ag: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		1Ag

1Ai ©

Specimen 1Ai: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		1Ai

1Ak ©

Specimen 1Ak: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1Ak

1Am ©

Specimen 1Am: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	1Am	Expression reduced.

1Ao ©

Specimen 1Ao: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1Ao

1Aq ©

Specimen 1Aq: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Trace	Single cells	1Aq	Expression barely detectable. Few expressing cells.

1Ca ©

Specimen 1Ca: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		1Ca

1Cc ©

Specimen 1Cc: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	1Cc	Expression in dorsal region,.

1Ce ©

Specimen 1Ce: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	1Ce	Expression restricted to posterior domain.

1Cg ©

Specimen 1Cg: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Weak	Regionally restricted	1Cg	Reduced expression in dorsal part.

6(I) a ©

Specimen 6(I)a: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(I) a
TS19: hindlimb bud	Present		6(I) a

6(I) c ©

Specimen 6(I)c: embryonic day 11.5; Six1^tm1Mair/Six1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(I) c
TS19: hindlimb bud	Weak	Regionally restricted	6(I) c	Expression severely reduced,

6(I) e ©

Specimen 6(I)e: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(I) e
TS19: hindlimb bud	Present		6(I) e

6(II) Myf5 PHL wt ©

Specimen 6(II) Myf5 PHL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6(II) Myf5 PHL wt

6(II) Myf5 PHL Six1-/- ©

Specimen 6(II) Myf5 PHL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	6(II) Myf5 PHL Six1-/-	Weak expressin in ventral aspect.

6(II) Myf5 PHL Six1-/-Six4-/+ ©

Specimen 6(II) Myf5 PHL Six1-/-Six4-/+: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Trace		6(II) Myf5 PHL Six1-/-Six4-/+	Expression barely detectable.

Specimen 6(II) Myf5 FL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(II) Myf5 FL wt

Specimen 6(II) Myf5 FL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(II) Myf5 FL Six1-/-	Expression absent in Six1-/- FL.

Specimen 6(III) Myf5 FL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		6(III) Myf5 FL wt

Specimen 6(III) Myf5 FL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Trace	Regionally restricted	6(III) Myf5 FL Six1-/-	Most expression lost except in posterior aspect.

Specimen 6(III) Myf5 PHL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present		6(III) Myf5 PHL wt

Specimen 6(III) Myf5 PHL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 PHL Six1-/-	Expression detected dorsally.

Specimen 6(III) Myf5 DHL wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 DHL wt	Expression detectable at 500 mm from tip of limb.

Specimen 6(III) Myf5 DHL Six1-/-: embryonic day 12.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	6(III) Myf5 DHL Six1-/-	Expression detectable at 900 mm from tip of limb.

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