Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36690
Buck CA, et al., Expression patterns of adhesion receptors in the developing mouse lung: functional implications. Cell Adhes Commun. 1996 Aug;4(2):69-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6504914
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Antibody:
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Anti-smooth muscle alpha-Actin (clone 1A4)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Embryos were staged according to Kaufman, M.H. (1992) The Atlas of Mouse Development. Academic Press, London.
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Results
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Specimen
2B:
embryonic day 10.0
(more  )
Note: Double labeled: green - Acta2; red - laminin.
Specimen
2B:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Acta2; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dorsal aorta |
Present |
Regionally restricted |
2B
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Expression is in smooth muscle.
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Specimen
2D:
embryonic day 12.5
(more )
Note: Double labeled: green - Acta2; red - laminin.
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Acta2; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung vascular element |
Present |
Regionally restricted |
2D
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Expression is in smooth muscle of the vessels in the lung.
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| TS20: smooth muscle tissue |
Present |
Regionally restricted |
2D
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Expression is in smooth muscle of the vessels in the lung.
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| TS20: bronchus |
Present |
Regionally restricted |
2D
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Expression is in smooth muscle in the trunk to the tip of the branch point.
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Specimen
2F:
embryonic day 14.0
(more )
Note: Double labeled: green - Acta2; red - laminin.
Specimen
2F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Acta2; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel smooth muscle |
Present |
Regionally restricted |
2F
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Expression is in smooth muscle of the vessels in the lung.
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| TS22: lung vascular element |
Present |
Regionally restricted |
2F
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Expression is in smooth muscle of the vessels in the lung.
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| TS22: bronchus |
Present |
Regionally restricted |
2F
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Expression is in smooth muscle in the trunk to the tip of the branch point.
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Specimen
4B:
embryonic day 14.0
(more )
Note: Double labeled: green - Acta2; red - laminin.
Specimen
4B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Acta2; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung vascular element |
Present |
Regionally restricted |
4B
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Expression is in smooth muscle of the vessels in the lung.
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| TS22: smooth muscle tissue |
Present |
Regionally restricted |
4B
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Expression is in smooth muscle of the vessels in the lung.
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| TS22: bronchus |
Present |
Regionally restricted |
4B
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Expression is in smooth muscle in the larger bronchi.
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| TS22: bronchiole |
Weak |
Regionally restricted |
4B
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Expression is absent in the termini of the budding bronchioles and is weak in the bronchial mesenchyme in the more distal pre-acinar bornchioles.
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