Probe:	Edn3 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: gut	Strong	Regionally restricted	3_10 dpc	Expression is in the vicinity of the developing caecum at the umbilical hernia.
TS17: forelimb bud mesenchyme	Weak		3_10 dpc

Structure	Level	Pattern	Image	Note
TS20: gut	Strong	Regionally restricted	3_12 dpc	Expression is in the vicinity of the developing caecum at the umbilical hernia.
TS20: forelimb mesenchyme	Weak		3_12 dpc

Structure	Level	Pattern	Image	Note
TS17: hindgut	Very strong	Regionally restricted	4a	Expression is in the caecum.
TS17: gut	Present	Graded	4a	There is a gradient of expression extending from the caecum into the proximal small intestine and distal colon.
TS17: stomach	Absent		4a

Structure	Level	Pattern	Image	Note
TS20: cecum	Very strong		4b
TS20: small intestine	Present	Graded	4b	There is a gradient of expression extending into the proximal small intestine.
TS20: large intestine	Present	Graded	4b	There is a gradient of expression extending into the distal colon.
TS20: stomach	Absent		4b

Structure	Level	Pattern	Image	Note
TS24: cecum	Very strong		4c
TS24: large intestine	Present		4c
TS24: small intestine	Present		4c
TS24: stomach	Absent		4c

Structure	Level	Pattern	Image	Note
TS20: cecum	Very strong	Regionally restricted	5	Expression is in the mesenchyme.
TS20: small intestine mesenchyme	Present		5
TS20: intestine mesenchyme	Present	Regionally restricted	5	Expression is in the mesenchyme of the colon.
TS20: large intestine	Present	Regionally restricted	5	Expression is in the mesenchyme of the colon.
TS20: intestine epithelium	Absent		5
TS20: liver	Absent		5
TS20: enteric nervous system	Absent		5	Expression is absent at the presumptive myenteric plexus.