Gene Expression Data
Assay Details
Assay
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Reference:
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J:66122
Miosge N, et al., Nidogen-1. Expression and ultrastructural localization during the onset of mesoderm formation in the early mouse embryo. J Histochem Cytochem. 2000 Feb;48(2):229-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6504039
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Gene symbol:
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Nid1
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Gene name:
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nidogen 1
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Antibody:
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JF4
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Detection system:
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Secondary antibody coupled to colloidal gold
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Results
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Specimen
2A:
embryonic day 7.5
(more )
Specimen
2A:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 7.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS11: Reichert's membrane |
Strong |
Regionally restricted |
2A
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Expressed in Reichert's membrane next to a parietal endoderm cell.
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Specimen
2B:
embryonic day 7.5
(more )
Specimen
2B:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 7.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS11: basement membrane |
Present |
Widespread |
2B
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Expressed over the entire extraembryonic basement membrane between the endoderm and yolk sac cells.
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Specimen
2C:
embryonic day 7.5
(more )
Specimen
2C:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 7.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: basement membrane |
Present |
Regionally restricted |
2C
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Expressed in the newly developed basement membrane zones between the ectodermal and endodermal cell layers.
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Specimen
2D:
embryonic day 7.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: basement membrane |
Present |
Regionally restricted |
2D
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Expressed in an ectodermal basement membrane within the embryo proper between the ectoderm and a mesodermal cell.
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Specimen
2E:
embryonic day 7.5
(more )
Specimen
2E:
(close )
Genetic Background:
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NMRI
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Age:
|
embryonic day 7.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: basement membrane |
Present |
Regionally restricted |
2E
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No staining was seen in a region at which a basement membrane was starting to develop.
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