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Gene Expression Data
Assay Details
Assay
Reference: J:37600 Puschel AW, et al., The sensory innervation of the mouse spinal cord may be patterned by differential expression of and differential responsiveness to semaphorins. Mol Cell Neurosci. 1996 May;7(5):419-31
Assay type: RNA in situ
MGI Accession ID: MGI:6504036
Gene symbol: Sema5b
Gene name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Probe: semG probe1
Probe preparation: labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen E11.5 not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present Expression mainly in ventricular zone.
TS19: neural tube marginal layer Present Expression begins to appear in marginal zone at E11.5

Specimen 2G: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord ventricular layer Present 2G Expression mainly in ventricular zone.
TS20: spinal cord marginal layer Present 2G

Specimen 4G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: spinal cord ventricular layer Present 4G
TS23: spinal cord gray matter Weak Regionally restricted 4G Lower level of expression mainly in gray matter of ventral spinal cord.
TS23: trunk connective tissue Present Regionally restricted 4G Expression in connective tissues separating individual muscle masses.

Specimen E12.5 not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: telencephalon Strong Regionally restricted Strong expression in neuroepitehlium of telencephalic vesicle.

Specimen 6G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: brain ventricular layer Present Widespread 6G Expression in ventricular zones of all ventricles.
TS23: cerebral cortex Present 6G Expression in cortical neuroepithelium.
TS23: spinal cord Present 6G Expression in neuroepithelium throughout out brain and spinal cord.

Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: brain Present Regionally restricted Authors report expression in distinct cell clusters in striatum, pallidum, thalamus and tegmentum.

Specimen 7D: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: telencephalon ventricular layer Present 7D
TS23: ganglionic eminence Present Regionally restricted 7D Expression in ventricular zone of ganglion eminence.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory