Probe:	semD probe1
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent			No expression in thoracic spinal cord at this stage.

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Present	Regionally restricted	1E	Expression in ventral half of thoracic spinal cord in both marginal and ventricular zones.
TS19: neural tube ventricular layer	Present	Regionally restricted	1E	Expression in ventral half of thoracic spinal cord in both marginal and ventricular zones.
TS19: vertebral pre-cartilage condensation	Present	Regionally restricted	1E	Authors report differential expression in prevertebrae.

Structure	Level	Pattern	Image	Note
TS20: spinal cord thoracic region	Strong	Regionally restricted	2D	Highly expressed in ventrolateral half of spinal cord.
TS20: spinal cord ventricular layer	Strong	Regionally restricted	2D	Highly expressed in band of cells in ventricular zone.
TS20: spinal cord ventral horn	Very strong		2D	Highest level of expression in ventral horn.

Structure	Level	Pattern	Image	Note
TS23: spinal cord thoracic region	Present	Regionally restricted	4D	Expression in ventral half of spinal cord.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted		Strong expression in neuroepithelium of telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS23: brain	Present	Regionally restricted	6D	Authors report expression in discrete regions in distinct groups of cells.
TS23: telencephalon ventricular layer	Absent		6D	Expression no longer detectable in telencephalic ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: brain	Present	Regionally restricted		Authors report expression in distinct cell clusters in striatum, pallidum, thalamus and tegmentum.