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Gene Expression Data
Assay Details
Assay
Reference: J:37600 Puschel AW, et al., The sensory innervation of the mouse spinal cord may be patterned by differential expression of and differential responsiveness to semaphorins. Mol Cell Neurosci. 1996 May;7(5):419-31
Assay type: RNA in situ
MGI Accession ID: MGI:6504031
Gene symbol: Sema4b
Gene name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Probe: semC probe2
Probe preparation: labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 1C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 1C Expression in post-otic hindbrain. Expression restricted to dorsal two-thirds of hindbrain.

Specimen E10.5 not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: future spinal cord Absent No expression in thoracic spinal cord at this stage.

Specimen 1D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present Regionally restricted 1D In thoracic region, expression first detectable at E11.5 at lateral margin of ventricular zone.
TS19: dorsal root ganglion Present 1D

Specimen 2C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord marginal layer Weak 2C
TS20: spinal cord lateral wall Strong Regionally restricted 2C Higher level of expression at dorsal boundary of ventricular and marginal zones.
TS20: dorsal root ganglion Present 2C

Specimen 4C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: back musculature Present Regionally restricted 4C Expression in epaxial muscles of back.
TS23: spinal cord thoracic region Present Widespread 4C Expression thoughout spinal cord with the exception of the superficial dorsal horn.
TS23: spinal cord dorsal horn Absent 4C
TS23: dorsal root ganglion Present 4C
TS23: sympathetic ganglion Present 4C

Specimen E12.5 not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brain Present Widespread Expression throughout brain in most postmitotic neurons.
TS20: telencephalon Weak Regionally restricted Weak expression in neuroepithelium of telencephalic vesicle.

Specimen 6C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: brain Present Uniform 6C Authors report expression was distributed more uniformly.
TS23: cerebral cortex Present 6C Low levels of expression in immature cortex.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory