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Gene Expression Data
Assay Details
Assay
Reference: J:135108 Manning JA, et al., Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development. Histochem Cell Biol. 2008 Jun;129(6):751-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6504011
Gene symbol: Tubg1
Gene name: tubulin, gamma 1
Antibody: anti-gamma-tubulin (GTU-88, T6557)
Assay notes: The specificity of the antibody used was not detailed, both family members have been annotated (Tubg1 and Tubg2).
Results
Specimen 4a: embryonic day 11.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 4a Merge , 4a g-tubulin Expression is in centrosomes.
TS19: dorsal root ganglion Present 4a Merge , 4a g-tubulin Expression is as a centrosomal spot or as weak difffuse cytoplasmic staining.

Specimen 4b: embryonic day 11.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 4b Nedd1/g-tubulin , 4b Merge , 4b g-tubulin Expression is in centrosomes.

Specimen 4c: embryonic day 11.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 4c Merge , 4c Nedd1/g-tubulin , 4c g-tubulin Expression is in centrosomes.
TS19: dorsal root ganglion Present 4c g-tubulin , 4c Merge , 4c Nedd1/g-tubulin Expression is as a centrosomal spot or as weak difffuse cytoplasmic staining.

Specimen 5a: embryonic day 11.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS19: lens vesicle epithelium Present Regionally restricted 5a Nedd1/g-tubulin , 5a Merge , 5a g-tubulin Expression is centrosomes in the apical region of polarized epithelial cells.

Specimen 5b: embryonic day 11.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS19: neural retina Present Regionally restricted 5b Nedd1/g-tubulin , 5b g-tubulin , 5b Merge Expression is at the subventricular region of polarized invaginated neural ectoderm cells.

Specimen 5c: embryonic day 14.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS22: lens epithelium Present Regionally restricted 5c g-tubulin , 5c Merge , 5c Nedd1/g-tubulin There is an apical polarized expression.

Specimen 5d: embryonic day 14.5 (more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Structure Level Pattern Image Note
TS22: neural retina Present 5d Nedd1/g-tubulin , 5d g-tubulin , 5d Merge Expression is in a polarized pattern in the subventricular region of cells of the outer neuroblastic layer.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory