Probe:	Emx1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		2C
TS20: cerebral cortex intermediate zone	Present		2C	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS20: cerebral cortex subventricular zone	Present		2C	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS20: cortical plate	Present		2C	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS20: cerebral cortex	Present	Regionally restricted	2C	Expression in migrating postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		2F
TS22: cerebral cortex intermediate zone	Present		2F	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS22: cerebral cortex subventricular zone	Present		2F	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS22: cortical plate	Present		2F	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		2I
TS24: cerebral cortex intermediate zone	Present		2I	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS24: cerebral cortex subventricular zone	Present		2I	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.
TS24: cortical plate	Present		2I	Expression in transitional field, comprising subventricular zone and intermediate zone, and cortical plate.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Widespread	3A	Expression in practically all cortical zones.
TS24: basal ganglia	Absent		3A	Anteroventral boundary of expression coinciding with boundary between cortex and basal ganglia.
TS24: diencephalon	Absent		3A	No expression detectable outside of telencephalic cortex.
TS24: hindbrain	Absent		3A	No expression detectable outside of telencephalic cortex.
TS24: midbrain	Absent		3A	No expression detectable outside of telencephalic cortex.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Widespread	3B	Expression in practically all cortical zones.

Structure	Level	Pattern	Image	Note
TS27: cortical subplate	Present	Regionally restricted	4A	Expression in specific neuronal populations of subplate.
TS27: neocortex layer	Present	Widespread	4A	Expression in practically all layers of cortical plate during postnatal development.
TS27: cortical layer I	Absent		4A	Expression excluded from layer 1.

Structure	Level	Pattern	Image	Note
TS28: cortical subplate	Present	Regionally restricted	4B	Expression in specific neuronal populations of subplate.
TS28: neocortex layer	Present	Widespread	4B	Expression in practically all layers of cortical plate during postnatal development.
TS28: cortical layer I	Absent		4B	Expression excluded from layer 1.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	5A	Authors report transient heavy reduction of expression in anterior-most regions of cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	5B	Stronger expression in more posterior regions. Anteroposterior boundary between strong and weaker expression.
TS27: main olfactory bulb glomerular layer	Present		5B	Expression in cells fated to give rise to mitral cell layer and glomerular layer of olfactory bulb.
TS27: main olfactory bulb mitral cell layer	Present		5B	Expression in cells fated to give rise to mitral cell layer and glomerular layer of olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5C	Authors report transient heavy reduction of expression in anterior-most regions of cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5D	Stronger expression in more posterior regions. Anteroposterior boundary between strong and weaker expression.
TS28: dentate gyrus	Present		5D
TS28: main olfactory bulb glomerular layer	Present		5D	Expression in cells fated to give rise to mitral cell layer and glomerular layer of olfactory bulb.
TS28: main olfactory bulb mitral cell layer	Present		5D	Expression in cells fated to give rise to mitral cell layer and glomerular layer of olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5E	Authors report transient heavy reduction of expression in anterior-most regions of cortex.
TS28: hippocampus	Present		5E

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5F	Stronger expression in more posterior regions.
TS28: hippocampus	Present		5F
TS28: main olfactory bulb glomerular layer	Present		5F

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5G	Anterioposterior differences not evident in adult.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5H	Anterioposterior differences not evident in adult.
TS28: hippocampus	Present		5H
TS28: main olfactory bulb glomerular layer	Present		5H

Structure	Level	Pattern	Image	Note
TS27: cingulate cortex	Weak		6A	Weaker expression in medial regions corresponding to cingulate cortex.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Strong		6B	Boundary between strong and weaker expression, coinciding with boundary between neocortex and limbic cortex.
TS27: cerebral cortex	Strong	Regionally restricted	6B	Strong expression in amygdaloid cortex.

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Weak		6C	Weaker expression in medial regions corresponding to cingulate cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	6D	Strong expression in amygdaloid cortex.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		6E

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Widespread	7A	Expression in practically every differentiating cell in cerebral cortex, to the exclusion of layer 2.
TS24: cortical subplate	Present	Regionally restricted	7A	Expression in part of subplate.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Widespread	7C	Expression in practically every differentiating cell in cerebral cortex, to the exclusion of layer 2.
TS26: cortical subplate	Present	Regionally restricted	7C	Expression in part of subplate.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	7E	Expression in practically every differentiating cell in cerebral cortex, to the exclusion of layer 2.
TS28: cortical layer II	Absent		7E
TS28: cortical subplate	Present		7E	Expression in part of subplate.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	8A
TS28: cortical layer V	Weak		8A	Expression consistenty weaker in layer 5.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present		8B	No difference in intensity of expression throughout layers.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present		8C	No difference in intensity of expression throughout layers.
TS28: cortical layer IV	Present		8C	No difference in intensity of expression throughout layers.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Weak	Regionally restricted	8D	Expression consistenty weaker in layer 5. Some neuronal nuclei or no expression.

Structure	Level	Pattern	Image	Note
TS28: cortical layer VI	Present		8E	No difference in intensity of expression throughout layers.

Structure	Level	Pattern	Image	Note
TS28: cortical subplate	Present	Regionally restricted	8F	Expression in some neurons of subplate.