Antibody:	anti-MATH1
Detection system:	Secondary antibody coupled to Texas Red

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1C	Expressed in a larger domain at the most dorsal aspect, and a small population of cells located at the lateral edges.
TS17: neural tube roof plate	Absent		1C

Structure	Level	Pattern	Image	Note
TS16: neural tube lateral wall	Present	Regionally restricted	1D	Expressed in a larger domain at the most dorsal aspect, and a small population of cells located at the lateral edges.
TS16: neural tube roof plate	Absent		1D

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	1E	Expressed in the external germinal layer.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present		2A	A subpopulation (30%) of positive-labeled cells co-localized with BrdU-labeled proliferating cells. Positive cells at the lateral edges did not incorporate BrdU.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	2B	A smaller number (20%) of positive-labeled cells co-localized with BrdU-labeled proliferating cells. Positive cells at the lateral edges did not incorporate BrdU.
TS19: neural tube ventricular layer	Present		2B	A smaller number (20%) of positive-labeled cells co-localized with BrdU-labeled proliferating cells.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present		2C	A subpopulation (30%) of positive-labeled cells co-localized with Tubb3. Co-expressing differentiating cells were found only at the lateral regions of the Atoh1+ domain.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present		2D	A larger percentage (40%) of positive-labeled cells co-localized with Tubb3.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	2E , 2G	Expressed exclusively in the proliferating (BrdU-positive) zone of the external germinal layer.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present		3A	A subset of positive-labeled cells at the lateral edges co-labeled with Cntn2.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present		3B	A subset of positive-labeled cells at the lateral edges co-labeled with Dcc.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3D	Expressed in a larger domain at the most dorsal aspect, and a small population of cells located at the lateral edges.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted		Expressed in a larger domain at the most dorsal aspect, and a small population of cells located at the lateral edges.
TS20: spinal cord roof plate	Absent