Gene Expression Data
Assay Details
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Assay
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Reference:
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J:163986
Bush JO, et al., Ephrin-B1 forward signaling regulates craniofacial morphogenesis by controlling cell proliferation across Eph-ephrin boundaries. Genes Dev. 2010 Sep 15;24(18):2068-80
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6501126
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Gene symbol:
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Ephb3
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Gene name:
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Eph receptor B3
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Probe:
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EphB3 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3D:
embryonic day 13.5
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
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3H
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EphB3 mRNA maintains wild-type expression pattern. No up-regulation of EphB3 mRNA in ephrin-B1-negative regions of mutant palates,
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Specimen
3L:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Absent |
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3L
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Expression not detectable in telencephalon at this stage.
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Specimen
S3D:
embryonic day 13.5; Efnb1tm1.1Sor/?
(more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed.
Specimen
S3D:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/?
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
Regionally restricted |
S3D
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Ephb3 mRNA expression very similar to Ephb3 protein expression
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Analysis Tools