Myh4 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:178848 An CI, et al., Genome-wide mapping of Sox6 binding sites in skeletal muscle reveals both direct and indirect regulation of muscle terminal differentiation by Sox6. BMC Dev Biol. 2011;11:59
Assay type:	RT-PCR
MGI Accession ID:	MGI:6491848
Gene symbol:	Myh4
Gene name:	myosin, heavy polypeptide 4, skeletal muscle

Probe:	Myh4-pE, Myh4-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR data are summarized in Table 1. Relative expression was normalized to beta-actin expression.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
TA 2	P m 2	TS28: tibialis anterior	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
TA 3	P m 3	TS28: tibialis anterior	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
TA 2	P m 2	TS28: tibialis anterior	Present (a)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
TA 3	P m 3	TS28: tibialis anterior	Present (b)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
EDL 2	P m 2	TS28: extensor digitorum longus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
EDL 3	P m 3	TS28: extensor digitorum longus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
EDL 2	P m 2	TS28: extensor digitorum longus	Present (c)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
EDL 3	P m 3	TS28: extensor digitorum longus	Present (b)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
Gas 2	P m 2	TS28: gastrocnemius muscle	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Gas 3	P m 3	TS28: gastrocnemius muscle	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Gas 2	P m 2	TS28: gastrocnemius muscle	Present (d)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
Gas 3	P m 3	TS28: gastrocnemius muscle	Present (e)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
Sol 2	P m 2	TS28: soleus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Sol 3	P m 3	TS28: soleus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Sol 2	P m 2	TS28: soleus	Present (f)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.
Sol 3	P m 3	TS28: soleus	Present (g)	Not Specified; total RNA	involves: 129 * 129S4/SvJaeSor	Myf5^tm3(cre)Sor/Myf5⁺, Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified	Conditional mutant.

Notes:
(a) 0.03 fold change in mRNA levels in the mutant skeletal muscle compared to control.
(b) 3 x 10<-3> fold change in mRNA levels in the mutant skeletal muscle compared to control.
(c) 0.20 fold change in mRNA levels in the mutant skeletal muscle compared to control.
(d) 0.12 fold change in mRNA levels in the mutant skeletal muscle compared to control.
(e) 2 x 10<-3> fold change in mRNA levels in the mutant skeletal muscle compared to control.
(f) 0.88 fold change in mRNA levels in the mutant skeletal muscle compared to control.
(g) 0.3 fold change in mRNA levels in the mutant skeletal muscle compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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