Vim Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:289397 Kowalkowski A, et al., Lack of discreet colocalization of epithelial apoptosis to the atretic precursor in the colon of the Fibroblast growth factor receptor 2IIIb mouse and staining consistent with cellular movement suggest a revised model of atresia formation. Dev Dyn. 2020 Jun;249(6):741-753
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6491434
Gene symbol:	Vim
Gene name:	vimentin

Antibody:	anti-vimentin (ab20346)
Detection system:	Alexa Fluor 488
Assay notes:	Fgfr2IIIb-/- (null) embryos were generated by mating Fgfr2IIIbflox/+; HprtCre/+ females with Fgfr2IIIbflox/flox males. Fgfr2IIIbflox/flox (control) embryos were generated within the same litters without the HPRT-Cre allele.

Results

8E ©

Specimen 8E: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Double labeled: red - Vim; green Cdh1 (assay MGI:6491431).

Structure	Level	Pattern	Image	Note
TS19: intestine epithelium	Absent		8E

8F ©

Specimen 8F: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Double labeled: red - Vim; green Cdh1 (assay MGI:6491431).

Structure	Level	Pattern	Image	Note
TS19: intestine	Present	Punctate	8F	Punctate expression in lumen of intestine.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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